| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Gli3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml (1), Western blot: 0 |
| Notes: |
1, There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form, This antibody will detect both, Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, depending on the testing material, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Gli3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P10071 |
| Purity: |
Antigen affinity |
| Description: |
190, Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), Gooseberry (gsb), PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein, The gene is expressed as an 8, The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation, a protein family that is known to regulate development in Drosophila, and Decapentaplegic (dpp) genes, and lung, and the protein product (relative molecular mass, myometrium, placenta, which regulates the Patched (pct), 000D) shows sequence-specific DNA binding, 5-kb mRNA in tissues such as testis, Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel |
| Immunogen: |
An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Gli3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Gli3 |
| Short name: |
Gli3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GLI family zinc finger 3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4207 |
| Gene: |
GCPS |
More about : GCPS |
| Long gene name: |
GLI family zinc finger 3 |
| Synonyms gene: |
PHS |
| Synonyms gene name: |
Greig cephalopolysyndactyly syndrome GLI-Kruppel family member GLI3 glioma-associated oncogene family zinc finger 3 |
| Synonyms: |
PAP-A PAPA PAPA1 PAPB ACLS PPDIV GLI3 |
| Synonyms name: |
zinc finger protein GLI3 oncogene GLI3 DNA-binding protein |
| Locus: |
7p14, 1 |
| Discovery year: |
1989-05-29 |
| Entrez gene record: |
2737 |
| Pubmed identfication: |
2118997 |
| RefSeq identity: |
NM_000168 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000023630 |