| Long name: |
Biotin Conjugated, EN2/Engrailed 2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
EN2/Engrailed 2 |
| Specificity: |
This is a highly specific antibody against EN2/Engrailed 2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
300-333/333 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human EN2/Engrailed 2 |
| Gene ID number: |
2020 |
| Swiss Prot: |
P19622 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Different mutations in the mouse homologs, During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region, EN-1 and EN-2, EN-1 and EN-2 regulate expression of a-synuclein, EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum, EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia, EN2, Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system, In Drosophila, a gene that is genetically linked to Parkinsonâ, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation, produce different developmental defects that frequently are lethal, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments, &euro, &trade, The engrailed-2 gene, s disease |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
EN2/Engrailed 2 Conjugated |
| Short name: |
Conjugated, EN2/Engrailed 2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, EN2/Engrailed 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3343 |
| Gene: |
EN2 |
More about : EN2 |
| Long gene name: |
engrailed homeobox 2 |
| Locus: |
7q36, 3 |
| Discovery year: |
1988-08-31 |
| Entrez gene record: |
2020 |
| RefSeq identity: |
NM_001427 |
| Classification: |
NKL subclass homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000151354 |