| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EN2/Engrailed 2 |
| Specificity: |
This antibody reacts specifically with EN2/Engrailed 2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EN2/Engrailed 2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Different mutations in the mouse homologs, During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region, EN-1 and EN-2, EN-1 and EN-2 regulate expression of a-synuclein, EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum, EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia, EN2, Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system, In Drosophila, a gene that is genetically linked to Parkinsonâ, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation, produce different developmental defects that frequently are lethal, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments, &euro, &trade, The engrailed-2 gene, s disease |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
EN2/Engrailed 2 Polyclonal Antibody |
| Other name: |
Anti-EN2/Engrailed 2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EN2/Engrailed 2 |
| Short name: |
Anti-EN2/Engrailed 2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EN2/Engrailed 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3343 |
| Gene: |
EN2 |
More about : EN2 |
| Long gene name: |
engrailed homeobox 2 |
| Locus: |
7q36, 3 |
| Discovery year: |
1988-08-31 |
| Entrez gene record: |
2020 |
| RefSeq identity: |
NM_001427 |
| Classification: |
NKL subclass homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000151354 |