| Long name: |
FITC Conjugated, Lefty Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Lefty |
| Specificity: |
This is a highly specific antibody against Lefty |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Lefty |
| Gene ID number: |
10637 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cleavage is therefore an essential step for Lefty activation, Complexes formed move into the nucleus, Lefty, Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor, Lefty also inhibits events downstream from R Smad phosphorylation, Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms), Lefty provides a repressed state of TGF beta responsive genes, Members of the TGF beta superfamily require processing for their activation, Secreted growth factors of the TGF beta family, TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells, TGF beta actions are directed by ligand induced activation of TGF beta receptors, The 28kDa form induces MAPK activity, The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, a left right axis is established, a novel member of the TGF beta superfamily, and Lefty A and Lefty B in humans, including gene products derived from nodal, inhibits TGF beta signaling, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries, where they act as components of a transcriptional complex, During vertebrate embryogenesis |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AI450052, Ebaf, FACTOR B, HOMOLOG OF antibody, LEFT-RIGHT DETERMINATION, LEFT-RIGHT DETERMINATION FACTOR 1, LEFTB, LEFTY 1, LEFTY B, LEFTY1, LEFTY1, LEFTY1, LEFTYB, Left right determination factor 1, Left right determination factor B, Lefty, MOUSE, Protein lefty-B, RGD1561867, STRA3, Stimulated by retinoic acid gene 3 protein, TGF beta 4, TGF-beta-4, Tgfb4, Transforming growth factor beta 4, Transforming growth factor beta-4, factor B, left right determination factor, left-right determination, left-right determination factor, lefty-1, Protein lefty-1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Lefty Conjugated |
| Short name: |
FITC Conjugated, Lefty Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Lefty (Antibody to) |
| Alternative technique: |
antibodies |