| Long name: |
FITC Conjugated, HSP27 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HSP27 |
| Specificity: |
This is a highly specific antibody against HSP27 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
100-150/205 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human HSP27 |
| Gene ID number: |
3315 |
| Swiss Prot: |
P04792 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins, Akt (PKB), Another complex consists of HSP27 and the IKK complex, Cytoplasmic HSP27 exists in multiple complexes, HSP27, HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, MAPKAP-kinase 2, One complex consists of HSP27, Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, The presence of HSP27 in this complex is required for Akt activation by stress stimuli, along with other members of the small HSP group, and p38 MAPK, and stimulates HSP27 binding to the IKK complex, possesses a C-terminal Alpha-crystalline homology domain, thereby inhibiting filament extension, where it may function to stabilize DNA and/or the nuclear membrane, Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HSP27 Conjugated |
| Short name: |
FITC Conjugated, HSP27 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HSP27 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5246 |
| Gene: |
HSPB1 |
More about : HSPB1 |
| Long gene name: |
heat shock protein family B (small) member 1 |
| Synonyms gene name: |
heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
X54079 |
| Entrez gene record: |
3315 |
| Pubmed identfication: |
2243808 9344682 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023228 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_248 |