| Long name: |
Cy5, 5 Conjugated, HSP27 (Ser82) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy5, 5, 5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HSP27 Ser82 |
| Specificity: |
This is a highly specific antibody against HSP27 Ser82 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser82 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Ser82 |
| Gene ID number: |
3315 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Because of the estrogen responsive nature of Hsp27, Hsp27 synthesis is induced by elevated temperature, Interestingly, MAP3K2/MEKK2, Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, This protein is involved in stress resistance and actin organization, This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors, [provided by RefSeq], also referred to as the Estrogen regulated 24K protein and HSP28, and MAP4K2/GCK, and environmental stresses, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, as well as estrogen in hormone responsive cells, but only one transcript variant has been supported and defined, endometrium and breast tissue, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein, is one of several small heat shock proteins (HSP) produced by all organisms studied, this protein has been studied extensively in human estrogen responsive tissues such as cervix, Hsp27, MAP3K3/MEKK5, The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
675nm/694nm |
| Synonyms: |
HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths |
| Gene target: |
HSP27 (Ser82) Conjugated |
| Short name: |
Conjugated, HSP27 (Ser82) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
5, Cy5 |
| Alternative name: |
cyanine 5, 5 coupled, HSP27 (Ser82) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5246 |
| Gene: |
HSPB1 |
More about : HSPB1 |
| Long gene name: |
heat shock protein family B (small) member 1 |
| Synonyms gene name: |
heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
X54079 |
| Entrez gene record: |
3315 |
| Pubmed identfication: |
2243808 9344682 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023228 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_248 |