| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Flag tag at the N-terminus, Recombinant Human D-glucuronyl C5-epimerase is produced by our Mammalian expression system and the target gene encoding Asn29-Asn617 is expressed with a 6His |
| Molecular Weight: |
68, 7 kD |
| UniProt number: |
O94923 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry Ice/ice packs |
| Formulation: |
2 um filtered solution of 20 mM Tris, 200 mM sodium chloride, Supplied as a 0, pH8 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
DYKDDDDKHHHHHHNKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
, Also separation of , Depending on the epitopes used human ELISA kits can be cross reactive to many other species, FLAGS are also used in the isolation of , For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies, Human proteins, It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray, Mainly analyzed are human serum, Modern , Suitable for in vivo use in cells, This FLAG-tags have the sequence DYKDDDDK motiv, because its mild purification procedure tends not to disrupt such complexes, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, or , or , overexpressed proteins from cell lysates is done by FLAG go HIS tags, plasma, primarily , saliva, urine, , (Homo sapiens, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, FLAG epitope that is a polypeptide , FLAG octapeptide, Homo sapiens sapiens), These tags are very useful to do protein purification by , affinity chromatography, humans , protein complexes , protein tag , recombinant, recombinant DNA, ssp, that can be added to a protein using , with multiple subunits |
| Conjugation: |
Flag |
| Group: |
recombinants |
| Gene target: |
D-glucuronyl C5-epimerase (N 6His) |
| Short name: |
Recombinant D-glucuronyl C5-epimerase (N- -6His) |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Label: |
Flag |
| Species: |
Humans, Human |
| Alternative name: |
sapiens D-glucuronyl complement component 5-epimerase (N-Flag-6His), recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
BT, C5 and IDBG-83167 and ENSG00000106804 and 727, C5a anaphylatoxin chemotactic receptor binding, C5a and C5b and C5D and CPAMD4 and ECLZB, Extracellular, Hc and IDBG-163835 and ENSMUSG00000026874 and 15139, alternative pathway and biological process this GO :0006958 and complement activation, classical pathway and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0008009 and chemokine activity and molecular function this GO :0010575 and positive regulation vascular endothelial growth factor production and biological process this GO :0010700 and negative regulation of norepinephrine secretion and biological process this GO :0010760 and negative regulation of macrophage chemotaxis and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0019835 and cytolysis and biological process this GO :0030449 and regulation of complement activation and biological process this GO :0031714 and C5a anaphylatoxin chemotactic receptor binding and molecular function this GO :0033602 and negative regulation of dopamine secretion and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0045087 and innate immune response and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0050921 and positive regulation of chemotaxis and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0090197 and positive regulation of chemokine secretion and biological process, this GO :0000187 and activation of MAPK activity and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0002523 and leukocyte migration involved in inflammatory response and biological process this GO :0004866 and endopeptidase inhibitor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005579 and membrane attack complex and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006874 and cellular calcium ion homeostasis and biological process this GO :0006935 and chemotaxis and biological process this GO :0006950 and response to stress and biological process this GO :0006954 and inflammatory response and biological process this GO :0006956 and complement activation and biological process this GO :0006957 and complement activation, this GO :0004866 : endopeptidase inhibitor activity, this GO :0004866 : endopeptidase inhibitor activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0008009 : chemokine activity and also this GO :0031714 : C5a anaphylatoxin chemotactic receptor binding, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0008009 : chemokine activity, this GO :0031714 : C5a anaphylatoxin chemotactic receptor binding, 24055 and IDBG-638834 and ENSBTAG00000012210 and 512045, complement component 5 |