| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Flag tag at the N-terminus, Recombinant Human Mothers Against Decapentaplegic Homolog 2 is produced by our E, coli expression system and the target gene encoding Ser2-Ser467 is expressed with a 6His |
| Molecular Weight: |
54, 6 kD |
| UniProt number: |
Q15796 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
pH7, 2 um filtered solution of 20 mM PB, 5, 500 mM sodium chloride, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MRGSHHHHHHGSDYKDDDDKSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
, Also separation of , Depending on the epitopes used human ELISA kits can be cross reactive to many other species, FLAGS are also used in the isolation of , For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies, Human proteins, It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray, Mainly analyzed are human serum, Modern , Suitable for in vivo use in cells, This FLAG-tags have the sequence DYKDDDDK motiv, because its mild purification procedure tends not to disrupt such complexes, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, or , or , overexpressed proteins from cell lysates is done by FLAG go HIS tags, plasma, primarily , saliva, urine, , (Homo sapiens, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, FLAG epitope that is a polypeptide , FLAG octapeptide, Homo sapiens sapiens), These tags are very useful to do protein purification by , affinity chromatography, humans , protein complexes , protein tag , recombinant, recombinant DNA, ssp, that can be added to a protein using , with multiple subunits |
| Conjugation: |
Flag |
| Group: |
recombinants |
| Gene target: |
SMAD2 (N-6His, Mothers Against Decapentaplegic Homolog 2 |
| Short name: |
SMAD2 (N-6His- ), Recombinant Mothers Against Decapentaplegic Homolog 2 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Label: |
Flag |
| Species: |
Humans, Human |
| Alternative name: |
SMAD family member 2 (N-6His-Flag), sapiens Mothers Against Decapentaplegic Homolog 2, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007182 and common-partner SMAD protein phosphorylation and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007352 and zy this GO tic specification of dorsal/ventral axis and biological process this GO :0007369 and gastrulation and biological process this GO :0007389 and pattern specification process and biological process this GO :0007492 and endoderm development and biological process this GO :0007507 and heart development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009749 and response to glucose and biological process this GO :0009791 and post-embryonic development and biological process this GO :0009880 and embryonic pattern specification and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0019902 and phosphatase binding and molecular function this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030073 and insulin secretion and biological process this GO :0030324 and lung development and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030513 and positive regulation of BMP signaling pathway and biological process this GO :0030618 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046332 and SMAD binding and molecular function this GO :0046872 and metal ion binding and molecular function this GO :0048340 and paraxial mesoderm morphogenesis and biological process this GO :0048589 and developmental growth and biological process this GO :0048617 and embryonic foregut morphogenesis and biological process this GO :0048701 and embryonic cranial skeleton morphogenesis and biological process this GO :0051098 and regulation of binding and biological process this GO :0060021 and palate development and biological process this GO :0060039 and pericardium development and biological process this GO :0070410 and co-SMAD binding and molecular function this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0070723 and response to cholesterol and biological process this GO :0071141 and SMAD protein complex and cellular component this GO :0071144 and SMAD2-SMAD3 protein complex and cellular component this GO :1900224 and positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry and biological process, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, nuclei, pathway-specific cytoplasmic mediator activity, pathway-specific cytoplasmic mediator activity and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0033613 : activating transcription factor binding and also this GO :0034713 : type I transforming growth factor beta receptor binding and also this GO :0035326 : enhancer binding and also this GO :0046332 : SMAD binding and also this GO :0046872 : metal ion binding and also this GO :0070410 : co-SMAD binding and also this GO :0070411 : I-SMAD binding and also this GO :0070412 : R-SMAD binding, pathway-specific cytoplasmic mediator activity and molecular function this GO :0031016 and pancreas development and biological process this GO :0031053 and primary miRNA processing and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032444 and activin responsive factor complex and cellular component this GO :0032924 and activin receptor signaling pathway and biological process this GO :0033613 and activating transcription factor binding and molecular function this GO :0034713 and type I transforming growth factor beta receptor binding and molecular function this GO :0035265 and organ growth and biological process this GO :0035326 and enhancer binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0038092 and nodal signaling pathway and biological process this GO :0043234 and protein complex and cellular component this GO :0045165 and cell fate commitment and biological process this GO :0045892 and negative regulation of transcription, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0001657 and ureteric bud development and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001706 and endoderm formation and biological process this GO :0001707 and mesoderm formation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005160 and transforming growth factor beta receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005160 : transforming growth factor beta receptor binding and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0019902 : phosphatase binding and also this GO :0030618 : transforming growth factor beta receptor, this GO :0003682 : chromatin binding, this GO :0003690 : double-stranded DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005160 : transforming growth factor beta receptor binding, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0019902 : phosphatase binding, this GO :0030618 : transforming growth factor beta receptor, this GO :0031625 : ubiquitin protein ligase binding, this GO :0033613 : activating transcription factor binding, this GO :0034713 : type I transforming growth factor beta receptor binding, this GO :0035326 : enhancer binding, this GO :0046332 : SMAD binding, this GO :0046872 : metal ion binding, this GO :0070410 : co-SMAD binding, this GO :0070411 : I-SMAD binding, this GO :0070412 : R-SMAD binding, transforming growth factor beta receptor, 516010, SMAD family member 2 |
| Identity: |
6768 |
| Gene: |
SMAD2 |
More about : SMAD2 |
| Long gene name: |
SMAD family member 2 |
| Synonyms gene: |
MADH2 |
| Synonyms gene name: |
MAD, mothers against DPP homolog 2 (Drosophila) , mothers against decapentaplegic homolog 2 (Drosophila) SMAD |
| Synonyms: |
MADR2 JV18-1 |
| Locus: |
18q21, 1 |
| Discovery year: |
1996-11-15 |
| GenBank acession: |
U65019 |
| Entrez gene record: |
4087 |
| Pubmed identfication: |
8752209 8673135 |
| RefSeq identity: |
NM_005901 |
| Classification: |
SMAD family |
| Havana BLAST/BLAT: |
OTTHUMG00000132652 |