| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Pyruvate kinase M2 is produced by our E, coli expression system and the target gene encoding Ser2-Pro531 is expressed with a 6His tag at the N-terminus |
| Molecular Weight: |
1 kD, 60 |
| UniProt number: |
P14618 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry ice/ice packs |
| Formulation: |
10% glycerol, pH7, 2 um filtered solution of phosphate buffered saline, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MGSSHHHHHHSSGLVPRGSHMSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
PKM2 (N-6His), Pyruvate Kinase M2 |
| Short name: |
PKM2 (N-6His), Recombinant Pyruvate Kinase M2 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
muscle (N-6His), pyruvate kinase, sapiens Pyruvate phosphorylation catalyst M2, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, muscle, nuclei, poly(A) RNA binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004743 and pyruvate kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005929 and cilium and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006096 and glycolytic process and biological process this GO :0012501 and programmed cell death and biological process this GO :0016310 and phosphorylation and biological process this GO :0023026 and MHC class II protein complex binding and molecular function this GO :0030955 and potassium ion binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0003824 : catalytic activity and also this GO :0004743 : pyruvate kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0023026 : MHC class II protein complex binding and also this GO :0030955 : potassium ion binding and also this GO :0044822 : poly(A) RNA binding, this GO :0003824 : catalytic activity, this GO :0004743 : pyruvate kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0023026 : MHC class II protein complex binding, this GO :0030955 : potassium ion binding, this GO :0044822 : poly(A) RNA binding, pyruvate kinase |
| Identity: |
9021 |
| Gene: |
PKM |
More about : PKM |
| Long gene name: |
muscle , pyruvate kinase |
| Synonyms gene: |
PKM2 |
| Synonyms: |
THBP1 OIP3 PK3 |
| Locus: |
15q23 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M23725 |
| Entrez gene record: |
5315 |
| Pubmed identfication: |
2040271 |
| Havana BLAST/BLAT: |
OTTHUMG00000172709 |