| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Pyruvate Kinase / PKM2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the PKM2 antibody should be determined by the researcher |
| Intented use: |
This PKM2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P14618 |
| Purity: |
Antigen affinity |
| Description: |
(1991) manipulated the intracellular Fru-1, (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides, 6-P2 concentration in several mammalian cell lines, 6-P2), 6-bisphosphate (Fru-1, Ashizawa et al, By in situ hybridization, Christofk et al, Muscle), Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25, The activity of pyruvate kinase subtype M2 is increased by fructose 1, Using a novel proteomic screen for phosphotyrosine-binding proteins, also known as PK3 or PKM2, by varying the glucose concentration in the media, including human, is an enzyme that in humans is encoded by the PKM gene, PKM (Pyruvate Kinase |
| Immunogen: |
Amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR of human PKM2 were used as the immunogen for the PKM2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PKM2 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Pyruvate Kinase / PKM2 |
| Short name: |
Pyruvate Kinase Antibody / PKM2 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
muscle, Pyruvate phosphorylation catalyst (Antibody to) / pyruvate kinase |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, muscle, nuclei, poly(A) RNA binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004743 and pyruvate kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005929 and cilium and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006096 and glycolytic process and biological process this GO :0012501 and programmed cell death and biological process this GO :0016310 and phosphorylation and biological process this GO :0023026 and MHC class II protein complex binding and molecular function this GO :0030955 and potassium ion binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0003824 : catalytic activity and also this GO :0004743 : pyruvate kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0023026 : MHC class II protein complex binding and also this GO :0030955 : potassium ion binding and also this GO :0044822 : poly(A) RNA binding, this GO :0003824 : catalytic activity, this GO :0004743 : pyruvate kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0023026 : MHC class II protein complex binding, this GO :0030955 : potassium ion binding, this GO :0044822 : poly(A) RNA binding, pyruvate kinase |
| Identity: |
9021 |
| Gene: |
PKM |
More about : PKM |
| Long gene name: |
muscle , pyruvate kinase |
| Synonyms gene: |
PKM2 |
| Synonyms: |
THBP1 OIP3 PK3 |
| Locus: |
15q23 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M23725 |
| Entrez gene record: |
5315 |
| Pubmed identfication: |
2040271 |
| Havana BLAST/BLAT: |
OTTHUMG00000172709 |