| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human SMAD4 is produced by our E, coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
5 kD, 61 |
| UniProt number: |
Q13485 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
2 um filtered solution of 20 mM TrisHCl，pH8, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLDLEHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
DPC4 (C-6His), SMAD4, SMAD Family Member 4 |
| Short name: |
DPC4 (C-6His), SMAD4, Recombinant SMAD Family Member 4 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
DPC4 (C-6His), SMAD family member 4, sapiens SMAD family Member 4, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007369 and gastrulation and biological process this GO :0007411 and axon guidance and biological process this GO :0007492 and endoderm development and biological process this GO :0007498 and mesoderm development and biological process this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0010862 and positive regulation of pathway-restricted SMAD protein phosphorylation and biological process this GO :0014033 and neural crest cell differentiation and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030509 and BMP signaling pathway and biological process this GO :0030511 and positive regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030513 and positive regulation of BMP signaling pathway and biological process this GO :0030616 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048589 and developmental growth and biological process this GO :0048663 and neuron fate commitment and biological process this GO :0048729 and tissue morphogenesis and biological process this GO :0048733 and sebaceous gland development and biological process this GO :0048859 and formation of anatomical boundary and biological process this GO :0051098 and regulation of binding and biological process this GO :0051797 and regulation of hair follicle development and biological process this GO :0060021 and palate development and biological process this GO :0060391 and positive regulation of SMAD protein import into nucleus and biological process this GO :0060395 and SMAD protein signal transduction and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060956 and endocardial cell differentiation and biological process this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0071141 and SMAD protein complex and cellular component this GO :0071559 and response to transforming growth factor beta and biological process this GO :0072133 and metanephric mesenchyme morphogenesis and biological process this GO :0072134 and nephrogenic mesenchyme morphogenesis and biological process, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, common-partner cytoplasmic mediator activity, common-partner cytoplasmic mediator activity and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding and also this GO :0070411 : I-SMAD binding and also this GO :0070412 : R-SMAD binding, common-partner cytoplasmic mediator activity and molecular function this GO :0032444 and activin responsive factor complex and cellular component this GO :0032525 and somite rostral/caudal axis specification and biological process this GO :0032909 and regulation of transforming growth factor beta2 production and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0036302 and atrioventricular canal development and biological process this GO :0042118 and endothelial cell activation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042177 and negative regulation of protein catabolic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045892 and negative regulation of transcription, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000988 and protein binding transcription factor activity and molecular function this GO :0001076 and RNA polymerase II transcription factor binding transcription factor activity and molecular function this GO :0001085 and RNA polymerase II transcription factor binding and molecular function this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001702 and gastrulation with mouth forming second and biological process this GO :0001822 and kidney development and biological process this GO :0003190 and atrioventricular valve formation and biological process this GO :0003198 and epithelial to mesenchymal transition involved in endocardial cushion formation and biological process this GO :0003251 and positive regulation of cell proliferation involved in heart valve morphogenesis and biological process this GO :0003279 and cardiac septum development and biological process this GO :0003360 and brainstem development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0000988 : protein binding transcription factor activity and also this GO :0001076 : RNA polymerase II transcription factor binding transcription factor activity and also this GO :0001085 : RNA polymerase II transcription factor binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0005518 : collagen binding and also this GO :0030616 : transforming growth factor beta receptor, this GO :0000988 : protein binding transcription factor activity, this GO :0001076 : RNA polymerase II transcription factor binding transcription factor activity, this GO :0001085 : RNA polymerase II transcription factor binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0005518 : collagen binding, this GO :0030616 : transforming growth factor beta receptor, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, this GO :0070411 : I-SMAD binding, this GO :0070412 : R-SMAD binding, transforming growth factor beta receptor, SMAD family member 4 |
| Identity: |
6770 |
| Gene: |
SMAD4 |
More about : SMAD4 |
| Long gene name: |
SMAD family member 4 |
| Synonyms gene: |
MADH4 |
| Synonyms gene name: |
MAD, mothers against DPP homolog 4 (Drosophila) , mothers against decapentaplegic homolog 4 (Drosophila) SMAD |
| Synonyms: |
DPC4 |
| Locus: |
18q21, 2 |
| Discovery year: |
1996-11-15 |
| GenBank acession: |
U44378 |
| Entrez gene record: |
4089 |
| Pubmed identfication: |
8553070 8774881 |
| RefSeq identity: |
NM_005359 |
| Classification: |
SMAD family |
| Havana BLAST/BLAT: |
OTTHUMG00000132696 |
| Locus Specific Databases: |
LRG_318 |