| Alternative name 1: |
60kD, Clone: SMD46 (DCS-46), DPC4, Mab anti-Human, WB/IF/IP (native &, denatured), SMAD4 |
| Alternative name 2: |
60kD, Clone: SMD46 (DCS-46), DPC4, Mab antibody-Human, WB/IF/IP (native &, denatured), SMAD4 |
| Alternative name 3: |
60kD, Clone: SMD46 (DCS-46), DPC4, Mab antibody-Homo sapiens, WB/IF/IP (native &, denatured), SMAD4 |
| Alternative name 4: |
denatured, anti-SMAD4 DPC4 60kD Clone: SMD46 DCS-46 / native & |
| Alternative name 5: |
denatured, SMAD4 DPC4 60kD Clone: SMD46 DCS-46 / native & |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| French translation: |
anticorps |
| Gene target: |
60kD, DPC4, SMD46 (DCS-46) Monoclonal /IF/IP (native denatured), SMAD4 |
| Short name: |
60kD, Clone: SMD46 (DCS-46) Mouse Monoclonal antibody- /IF/IP (native denatured), DPC4, SMAD4 |
| Technique: |
Monoclonals or monoclonal antibodies, mouses, Mouse |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
60kD, DPC4, Mouse monoclonal (Antibody to)-H, Western Blot/IF/IP (native &, clonality: SMD46 (DCS-46), denaturation), sapiens, SMAD family member 4 |
| Alternative technique: |
murine |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007369 and gastrulation and biological process this GO :0007411 and axon guidance and biological process this GO :0007492 and endoderm development and biological process this GO :0007498 and mesoderm development and biological process this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0010862 and positive regulation of pathway-restricted SMAD protein phosphorylation and biological process this GO :0014033 and neural crest cell differentiation and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030509 and BMP signaling pathway and biological process this GO :0030511 and positive regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030513 and positive regulation of BMP signaling pathway and biological process this GO :0030616 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048589 and developmental growth and biological process this GO :0048663 and neuron fate commitment and biological process this GO :0048729 and tissue morphogenesis and biological process this GO :0048733 and sebaceous gland development and biological process this GO :0048859 and formation of anatomical boundary and biological process this GO :0051098 and regulation of binding and biological process this GO :0051797 and regulation of hair follicle development and biological process this GO :0060021 and palate development and biological process this GO :0060391 and positive regulation of SMAD protein import into nucleus and biological process this GO :0060395 and SMAD protein signal transduction and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060956 and endocardial cell differentiation and biological process this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0071141 and SMAD protein complex and cellular component this GO :0071559 and response to transforming growth factor beta and biological process this GO :0072133 and metanephric mesenchyme morphogenesis and biological process this GO :0072134 and nephrogenic mesenchyme morphogenesis and biological process, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, common-partner cytoplasmic mediator activity, common-partner cytoplasmic mediator activity and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding and also this GO :0070411 : I-SMAD binding and also this GO :0070412 : R-SMAD binding, common-partner cytoplasmic mediator activity and molecular function this GO :0032444 and activin responsive factor complex and cellular component this GO :0032525 and somite rostral/caudal axis specification and biological process this GO :0032909 and regulation of transforming growth factor beta2 production and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0036302 and atrioventricular canal development and biological process this GO :0042118 and endothelial cell activation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042177 and negative regulation of protein catabolic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045892 and negative regulation of transcription, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000988 and protein binding transcription factor activity and molecular function this GO :0001076 and RNA polymerase II transcription factor binding transcription factor activity and molecular function this GO :0001085 and RNA polymerase II transcription factor binding and molecular function this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001702 and gastrulation with mouth forming second and biological process this GO :0001822 and kidney development and biological process this GO :0003190 and atrioventricular valve formation and biological process this GO :0003198 and epithelial to mesenchymal transition involved in endocardial cushion formation and biological process this GO :0003251 and positive regulation of cell proliferation involved in heart valve morphogenesis and biological process this GO :0003279 and cardiac septum development and biological process this GO :0003360 and brainstem development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0000988 : protein binding transcription factor activity and also this GO :0001076 : RNA polymerase II transcription factor binding transcription factor activity and also this GO :0001085 : RNA polymerase II transcription factor binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0005518 : collagen binding and also this GO :0030616 : transforming growth factor beta receptor, this GO :0000988 : protein binding transcription factor activity, this GO :0001076 : RNA polymerase II transcription factor binding transcription factor activity, this GO :0001085 : RNA polymerase II transcription factor binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0005518 : collagen binding, this GO :0030616 : transforming growth factor beta receptor, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, this GO :0070411 : I-SMAD binding, this GO :0070412 : R-SMAD binding, transforming growth factor beta receptor, SMAD family member 4 |
| Identity: |
6770 |
| Gene: |
SMAD4 |
More about : SMAD4 |
| Long gene name: |
SMAD family member 4 |
| Synonyms gene: |
MADH4 |
| Synonyms gene name: |
MAD, mothers against DPP homolog 4 (Drosophila) , mothers against decapentaplegic homolog 4 (Drosophila) SMAD |
| Synonyms: |
DPC4 |
| Locus: |
18q21, 2 |
| Discovery year: |
1996-11-15 |
| GenBank acession: |
U44378 |
| Entrez gene record: |
4089 |
| Pubmed identfication: |
8553070 8774881 |
| RefSeq identity: |
NM_005359 |
| Classification: |
SMAD family |
| Havana BLAST/BLAT: |
OTTHUMG00000132696 |
| Locus Specific Databases: |
LRG_318 |