| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Lin28a |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000-2000 |
| Notes: |
Titration of the Lin28a antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Lin28a antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q8K3Y3 |
| Purity: |
Antigen affinity |
| Description: |
ARBP/36B4 ribosomal protein mRNA and its own mRNA, Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'- GGAG-3' motif in the terminal loop, Binds IGF2 mRNA, Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells, Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression, In contrast, Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, Lin28a down-regulation in neural stem cells by miR-125, MYOD1 mRNA, Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7, Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation, a miRNA precursor, allows the processing of pre-let-7, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis, in stabilizing the mRNAs, indirectly, leading to the terminal uridylation of pre- let-7, leading to their terminal uridylation and subsequent degradation, Acts as a 'translational enhancer' |
| Immunogen: |
This mouse Lin28a antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 177-210 amino acids from the C-terminal region of mouse Lin28a |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Lin28a antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Lin28a |
| Short name: |
Lin28a Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Lin28a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
38056 |
| Gene: |
LIN28AP2 |
More about : LIN28AP2 |
| Long gene name: |
LIN28A pseudogene 2 |
| Synonyms gene name: |
elegans) pseudogene 2 , lin-28 homolog A (C |
| Locus: |
13q31, 2 |
| Discovery year: |
2010-04-06 |
| Entrez gene record: |
100287388 |
| Havana BLAST/BLAT: |
OTTHUMG00000017164 |