| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LIN28A |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the LIN28A antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LIN28A antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9H9Z2 |
| Purity: |
Antigen affinity |
| Description: |
ARBP/36B4 ribosomal protein mRNA and its own mRNA, Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, Binds IGF2 mRNA, Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells, Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 [removed]By similarity), In contrast, Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, LIN28A down-regulation in neural stem cells by miR-125, MYOD1 mRNA, Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7, Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation, a miRNA precursor, allows the processing of pre-let-7, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis, in stabilizing the mRNAs, indirectly, leading to the terminal uridylation of pre-let-7, leading to their terminal uridylation and subsequent degradation, Acts as a 'translational enhancer' |
| Immunogen: |
This LIN28A antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the N-terminal region of human LIN28A |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LIN28A antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LIN28A |
| Short name: |
LIN28A Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LIN28A (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
15986 |
| Gene: |
LIN28A |
More about : LIN28A |
| Long gene name: |
lin-28 homolog A |
| Synonyms gene: |
LIN28 |
| Synonyms gene name: |
elegans) , lin-28 homolog (C |
| Synonyms: |
LIN-28 FLJ12457 ZCCHC1 CSDD1 |
| Locus: |
1p36, 11 |
| Discovery year: |
2003-07-21 |
| GenBank acession: |
AF521099 |
| Entrez gene record: |
79727 |
| Pubmed identfication: |
12798299 |
| RefSeq identity: |
NM_024674 |
| Classification: |
Zinc fingers CCHC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000003550 |