| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
UHRF1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:4000 |
| Notes: |
Titration of the UHRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This UHRF1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q96T88 |
| Purity: |
Antigen affinity |
| Description: |
Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications, Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication, Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML, In addition to its role in maintenance of DNA methylation, It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo, May be involved in DNA repair, Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, and recruits chromatin proteins, respectively, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification |
| Immunogen: |
This UHRF1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 592-626 amino acids from the N-terminal region of human UHRF1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the UHRF1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
UHRF1 |
| Short name: |
UHRF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
UHRF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12556 |
| Gene: |
UHRF1 |
More about : UHRF1 |
| Long gene name: |
ubiquitin like with PHD and ring finger domains 1 |
| Synonyms: |
ICBP90 Np95 FLJ21925 RNF106 TDRD22 |
| Locus: |
19p13, 3 |
| Discovery year: |
2000-03-15 |
| GenBank acession: |
AF129507 |
| Entrez gene record: |
29128 |
| Pubmed identfication: |
10646863 |
| RefSeq identity: |
NM_001048201 |
| Classification: |
PHD finger proteins Ring finger proteins Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000180251 |