| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LKB1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, WB, IHC |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the LKB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LKB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q15831 |
| Purity: |
Purified |
| Description: |
Able to phosphorylate p53/TP53, Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, Acts by phosphorylating the T-loop of AMPK family proteins, Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Also phosphorylates non-AMPK family proteins such as STRADA, BRSK1, BRSK2, In association with NUAK1, In vein endothelial cells, Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, PRKAA2, PTEN and possibly p53/TP53, Regulates UV radiation-induced DNA damage response mediated by CDKN1A, Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, SIK1, SIK2, SIK3 and SNRK but not MELK, [UniProt], activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage, apoptosis and DNA damage response, cell polarity, glucose homeostasis in liver, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro), leading to axon initiation and specification, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1, thereby playing a role in various processes such as cell metabolism, thus promoting their activity: phosphorylates PRKAA1, LKB1 is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members |
| Immunogen: |
A portion of amino acids 14-44 from the human protein was used as the immunogen for this LKB1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LKB1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LKB1 |
| Short name: |
LKB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LKB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11389 |
| Gene: |
STK11 |
More about : STK11 |
| Long gene name: |
serine/threonine kinase 11 |
| Synonyms gene name: |
serine/threonine kinase 11 (Peutz-Jeghers syndrome) |
| Synonyms: |
PJS LKB1 |
| Synonyms name: |
polarization-related protein LKB1 |
| Locus: |
19p13, 3 |
| Discovery year: |
1998-01-21 |
| GenBank acession: |
U63333 |
| Entrez gene record: |
6794 |
| Pubmed identfication: |
9425897 |
| RefSeq identity: |
NM_000455 |
| Havana BLAST/BLAT: |
OTTHUMG00000180118 |
| Locus Specific Databases: |
LRG_319 |