| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
VDAC |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the VDAC antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This VDAC antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P21796 |
| Purity: |
Antigen affinity |
| Description: |
It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules, The open state has a weak anion selectivity whereas the closed state is cation-selective, The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis, in the plasma membrane it is involved in cell volume regulation and apoptosis, VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane |
| Immunogen: |
A portion of amino acids 1-30 from human VDAC1 was used as the immunogen for this VDAC antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the VDAC antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
VDAC |
| Short name: |
VDAC Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
VDAC (Antibody to) |
| Alternative technique: |
antibodies |