| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SMAD6 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, FACS |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the SMAD6 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SMAD6 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O43541 |
| Purity: |
Antigen affinity |
| Description: |
Binds to regulatory elements in target promoter regions, May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding, Suppresses IL1R-TLR signaling through its direct interaction with PEL1, [UniProt], nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes, preventing NF-kappa-B activation, SMAD6 acts as a mediator of TGF-beta and BMP antiflammatory activity |
| Immunogen: |
A portion of amino acids 358-386 from the human protein was used as the immunogen for this SMAD6 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot the SMAD6 antibody and store frozen at -20 deg, Store at 4oC for up to one month |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SMAD6 |
| Short name: |
SMAD6 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SMAD family member 6 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AOVD2 and HsT17432 and MADH6 and MADH7, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006955 and immune response and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007352 and zy this GO tic specification of dorsal/ventral axis and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010991 and negative regulation of SMAD protein complex assembly and biological process this GO :0030509 and BMP signaling pathway and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0030617 and transforming growth factor beta receptor, SMAD6 and IDBG-18304 and ENSG00000137834 and 4091, SMAD6 and IDBG-645465 and ENSBTAG00000000625 and 511279, Smad6 and IDBG-175757 and ENSMUSG00000036867 and 17130, inhibitory cytoplasmic mediator activity, inhibitory cytoplasmic mediator activity and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0034713 : type I transforming growth factor beta receptor binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding and also this GO :0070410 : co-SMAD binding and also this GO :0070411 : I-SMAD binding and also this GO :0070412 : R-SMAD binding and also this GO :0070698 : type I activin receptor binding, inhibitory cytoplasmic mediator activity and molecular function this GO :0031589 and cell-substrate adhesion and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0034616 and response to laminar fluid shear stress and biological process this GO :0034713 and type I transforming growth factor beta receptor binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0043627 and response to estrogen and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045444 and fat cell differentiation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0060394 and negative regulation of pathway-restricted SMAD protein phosphorylation and biological process this GO :0070410 and co-SMAD binding and molecular function this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0070698 and type I activin receptor binding and molecular function, nuclei, this GO :0001657 and ureteric bud development and biological process this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0003682 : chromatin binding, this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0030617 : transforming growth factor beta receptor, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0030617 : transforming growth factor beta receptor, this GO :0031625 : ubiquitin protein ligase binding, this GO :0034713 : type I transforming growth factor beta receptor binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, this GO :0070410 : co-SMAD binding, this GO :0070411 : I-SMAD binding, this GO :0070412 : R-SMAD binding, this GO :0070698 : type I activin receptor binding, transforming growth factor beta receptor, SMAD family member 6 |
| Identity: |
6772 |
| Gene: |
SMAD6 |
More about : SMAD6 |
| Long gene name: |
SMAD family member 6 |
| Synonyms gene: |
MADH7 MADH6 |
| Synonyms gene name: |
MAD, mothers against DPP homolog 6 (Drosophila) , mothers against decapentaplegic homolog 6 (Drosophila) SMAD |
| Synonyms: |
HsT17432 |
| Locus: |
15q22, 31 |
| Discovery year: |
1997-08-22 |
| GenBank acession: |
BC012986 |
| Entrez gene record: |
4091 |
| Pubmed identfication: |
9256479 |
| RefSeq identity: |
NM_005585 |
| Classification: |
SMAD family |
| Havana BLAST/BLAT: |
OTTHUMG00000133218 |
| Locus Specific Databases: |
The Globin Gene Server |