| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NPM1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB, FACS |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the NPM1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This NPM1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P06748 |
| Purity: |
Purified |
| Description: |
It is implicated in multiple functions, NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia, and regulation of the tumor suppressor ARF, control of centrosome duplication, including ribosomal protein assembly and transport, Nucleophosmin/NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm |
| Immunogen: |
A portion of amino acids 198-226 from the human protein was used as the immunogen for this NPM1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the NPM1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NPM1 |
| Short name: |
NPM1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
numatrin) (Antibody to), nucleophosmin (nucleolar phosphoprotein B23 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
B23 and NPM, NPM1 and IDBG-57453 and ENSG00000181163 and 4869, nuclei, numatrin), this GO :0003676 and nucleic acid binding and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0004860 and protein kinase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006281 and DNA repair and biological process this GO :0006334 and nucleosome assembly and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006913 and nucleocytoplasmic transport and biological process this GO :0006950 and response to stress and biological process this GO :0007098 and centrosome cycle and biological process this GO :0007165 and signal transduction and biological process this GO :0007569 and cell aging and biological process this GO :0008104 and protein localization and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010826 and negative regulation of centrosome duplication and biological process this GO :0016020 and membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030529 and ribonucleoprotein complex and cellular component this GO :0030957 and Tat protein binding and molecular function this GO :0031616 and spindle pole centrosome and cellular component this GO :0032071 and regulation of endodeoxyribonuclease activity and biological process this GO :0034080 and centromere-specific nucleosome assembly and biological process this GO :0042255 and ribosome assembly and biological process this GO :0042393 and histone binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043023 and ribosomal large subunit binding and molecular function this GO :0043024 and ribosomal small subunit binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044387 and negative regulation of protein kinase activity by regulation of protein phosphorylation and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045727 and positive regulation of translation and biological process this GO :0046599 and regulation of centriole replication and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0051059 and NF-kappaB binding and molecular function this GO :0051082 and unfolded protein binding and molecular function this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051259 and protein oli this GO merization and biological process this GO :0060699 and regulation of endoribonuclease activity and biological process this GO :0060735 and regulation of eIF2 alpha phosphorylation by dsRNA and biological process, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003713 : transcription coactivator activity and also this GO :0003723 : RNA binding and also this GO :0004860 : protein kinase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding and also this GO :0030957 : Tat protein binding and also this GO :0042393 : histone binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043023 : ribosomal large subunit binding and also this GO :0043024 : ribosomal small subunit binding and also this GO :0044822 : poly(A) RNA binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0051059 : NF-kappaB binding and also this GO :0051082 : unfolded protein binding, this GO :0003713 : transcription coactivator activity, this GO :0003723 : RNA binding, this GO :0004860 : protein kinase inhibitor activity, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, this GO :0030957 : Tat protein binding, this GO :0042393 : histone binding, this GO :0042803 : protein homodimerization activity, this GO :0043023 : ribosomal large subunit binding, this GO :0043024 : ribosomal small subunit binding, this GO :0044822 : poly(A) RNA binding, this GO :0046982 : protein heterodimerization activity, this GO :0051059 : NF-kappaB binding, this GO :0051082 : unfolded protein binding, unfolded protein binding, nucleophosmin (nucleolar phosphoprotein B23 |
| Identity: |
7910 |
| Gene: |
NPM1 |
More about : NPM1 |
| Long gene name: |
nucleophosmin |
| Synonyms gene name: |
nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Synonyms: |
B23 NPM |
| Synonyms name: |
member 1 , nucleolar phosphoprotein B23 numatrin nucleophosmin/nucleoplasmin family |
| Locus: |
5q35, 1 |
| Discovery year: |
1993-08-24 |
| GenBank acession: |
M26697 |
| Entrez gene record: |
4869 |
| Pubmed identfication: |
8471164 8122112 |
| RefSeq identity: |
NM_002520 |
| Havana BLAST/BLAT: |
OTTHUMG00000130465 |
| Locus Specific Databases: |
LRG_458 |