| Long name: |
1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Also known as: |
1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
NPM1 |
| Specificity: |
This is a highly specific antibody against NPM1 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
1, 1042CT5, 2 |
| Source: |
Purified His-tagged NPM1 protein was used to produced this monoclonal antibody |
| Gene ID number: |
4869 |
| Swiss Prot: |
P06748 |
| Tested Applications: |
IHC-P, WB |
| Recommended dilutions: |
WB(1:1000), IHC-P(1:25) |
| Cross reactivity: |
Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Acts as a chaperonin for the core histones H3, Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486), Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids, Binds ribosome presumably to drive ribosome nuclear export, H2B and H4, In concert with BRCA2, May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules, Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation, Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication, Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA, and regulation of tumor suppressors p53/TP53 and ARF, cell proliferation, centrosome duplication, histone assembly, protein chaperoning, regulates centrosome duplication, Involved in diverse cellular processes such as ribosome biogenesis |
| Purification method: |
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin, B23 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
1) Monoclonal, 2, NPM1 (1042CT5 |
| Short name: |
1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
numatrin) (1042CT5, 1) monoclonal (Antibody to), 2, nucleophosmin (nucleolar phosphoprotein B23 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
B23 and NPM, NPM1 and IDBG-57453 and ENSG00000181163 and 4869, nuclei, numatrin), this GO :0003676 and nucleic acid binding and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0004860 and protein kinase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006281 and DNA repair and biological process this GO :0006334 and nucleosome assembly and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006913 and nucleocytoplasmic transport and biological process this GO :0006950 and response to stress and biological process this GO :0007098 and centrosome cycle and biological process this GO :0007165 and signal transduction and biological process this GO :0007569 and cell aging and biological process this GO :0008104 and protein localization and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010826 and negative regulation of centrosome duplication and biological process this GO :0016020 and membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030529 and ribonucleoprotein complex and cellular component this GO :0030957 and Tat protein binding and molecular function this GO :0031616 and spindle pole centrosome and cellular component this GO :0032071 and regulation of endodeoxyribonuclease activity and biological process this GO :0034080 and centromere-specific nucleosome assembly and biological process this GO :0042255 and ribosome assembly and biological process this GO :0042393 and histone binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043023 and ribosomal large subunit binding and molecular function this GO :0043024 and ribosomal small subunit binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044387 and negative regulation of protein kinase activity by regulation of protein phosphorylation and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045727 and positive regulation of translation and biological process this GO :0046599 and regulation of centriole replication and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0051059 and NF-kappaB binding and molecular function this GO :0051082 and unfolded protein binding and molecular function this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051259 and protein oli this GO merization and biological process this GO :0060699 and regulation of endoribonuclease activity and biological process this GO :0060735 and regulation of eIF2 alpha phosphorylation by dsRNA and biological process, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003713 : transcription coactivator activity and also this GO :0003723 : RNA binding and also this GO :0004860 : protein kinase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding and also this GO :0030957 : Tat protein binding and also this GO :0042393 : histone binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043023 : ribosomal large subunit binding and also this GO :0043024 : ribosomal small subunit binding and also this GO :0044822 : poly(A) RNA binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0051059 : NF-kappaB binding and also this GO :0051082 : unfolded protein binding, this GO :0003713 : transcription coactivator activity, this GO :0003723 : RNA binding, this GO :0004860 : protein kinase inhibitor activity, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, this GO :0030957 : Tat protein binding, this GO :0042393 : histone binding, this GO :0042803 : protein homodimerization activity, this GO :0043023 : ribosomal large subunit binding, this GO :0043024 : ribosomal small subunit binding, this GO :0044822 : poly(A) RNA binding, this GO :0046982 : protein heterodimerization activity, this GO :0051059 : NF-kappaB binding, this GO :0051082 : unfolded protein binding, unfolded protein binding, nucleophosmin (nucleolar phosphoprotein B23 |
| Identity: |
7910 |
| Gene: |
NPM1 |
More about : NPM1 |
| Long gene name: |
nucleophosmin |
| Synonyms gene name: |
nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Synonyms: |
B23 NPM |
| Synonyms name: |
member 1 , nucleolar phosphoprotein B23 numatrin nucleophosmin/nucleoplasmin family |
| Locus: |
5q35, 1 |
| Discovery year: |
1993-08-24 |
| GenBank acession: |
M26697 |
| Entrez gene record: |
4869 |
| Pubmed identfication: |
8471164 8122112 |
| RefSeq identity: |
NM_002520 |
| Havana BLAST/BLAT: |
OTTHUMG00000130465 |
| Locus Specific Databases: |
LRG_458 |