| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
RIG-I (DDX58) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the RIG-I antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This RIG-I antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95786 |
| Purity: |
Purified |
| Description: |
IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5), It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses, Japanese encephalitis virus and HCV, RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD), RIG-I triggers a transduction cascade involving MAVS/IPS1, This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, Upon interaction with intracellular dsRNA produced during viral replication, are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), influenza viruses, which results in the activation of NF-kappa-B, DEAD box proteins |
| Immunogen: |
A portion of amino acids 894-925 from the human protein was used as the immunogen for this RIG-I antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the RIG-I antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
RIG-I (DDX58) |
| Short name: |
RIG-I Antibody (DDX58) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
RIG-I (Antibody to) (DEAD (L-aspartic acid-L-gutamic acid-alanine-L-aspartic acid) box polypeptide 58) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cell surfaces, DDX58 and IDBG-55854 and ENSG00000107201 and 23586, DDX58 and IDBG-629773 and ENSBTAG00000003366 and 504760, Ddx58 and IDBG-135957 and ENSMUSG00000040296 and 230073, RIG-I and RIGI and RLR-1, acting on acid anhydrides, acting on acid anhydrides and also this GO :0042802 : identical protein binding, acting on acid anhydrides and molecular function this GO :0030334 and regulation of cell migration and biological process this GO :0032480 and negative regulation of type I interferon production and biological process this GO :0032587 and ruffle membrane and cellular component this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0034344 and regulation of type III interferon production and biological process this GO :0039528 and cytoplasmic pattern recognition receptor signaling pathway in response to virus and biological process this GO :0039529 and RIG-I signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042993 and positive regulation of transcription factor import into nucleus and biological process this GO :0043330 and response to exogenous dsRNA and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process, hydrolase activity, this GO :0002230 and positive regulation of defense response to virus by host and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003725 and double-stranded RNA binding and molecular function this GO :0003727 and single-stranded RNA binding and molecular function this GO :0004386 and helicase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0008152 and metabolic process and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009597 and detection of virus and biological process this GO :0009615 and response to virus and biological process this GO :0015629 and actin cytoskeleton and cellular component this GO :0016032 and viral process and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016817 and hydrolase activity, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003677 : DNA binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003725 : double-stranded RNA binding and also this GO :0003727 : single-stranded RNA binding and also this GO :0004386 : helicase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008270 : zinc ion binding and also this GO :0016787 : hydrolase activity and also this GO :0016817 : hydrolase activity, this GO :0003677 : DNA binding, this GO :0003690 : double-stranded DNA binding, this GO :0003725 : double-stranded RNA binding, this GO :0003727 : single-stranded RNA binding, this GO :0004386 : helicase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008270 : zinc ion binding, this GO :0016787 : hydrolase activity, this GO :0016817 : hydrolase activity, this GO :0042802 : identical protein binding, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Identity: |
19102 |
| Gene: |
DDX58 |
More about : DDX58 |
| Long gene name: |
DExD/H-box helicase 58 |
| Synonyms gene name: |
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Synonyms: |
RIG-I FLJ13599 DKFZp434J1111 |
| Synonyms name: |
RNA helicase RIG-I retinoic acid inducible gene I |
| Locus: |
9p21, 1 |
| Discovery year: |
2004-05-10 |
| GenBank acession: |
AF038963 |
| Entrez gene record: |
23586 |
| Pubmed identfication: |
21690088 |
| RefSeq identity: |
NM_014314 |
| Classification: |
DEAD-box helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000019746 |