| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ATG7 (APG7) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the APG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This APG7 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95352 |
| Purity: |
Purified |
| Description: |
APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE), APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3, APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12, Formation of the PE conjugate is essential for autophagy, Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole), Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells |
| Immunogen: |
A portion of amino acids 284-313 from the human protein was used as the immunogen for this APG7 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the APG7 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ATG7 (APG7) |
| Short name: |
ATG7 Antibody (APG7) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ATG7 (Antibody to) (APG7) |
| Alternative technique: |
antibodies |
| Identity: |
16935 |
| Gene: |
ATG7 |
More about : ATG7 |
| Long gene name: |
autophagy related 7 |
| Synonyms gene: |
APG7L |
| Synonyms gene name: |
APG7 autophagy 7-like (S, cerevisiae) , cerevisiae) ATG7 autophagy related 7 homolog (S |
| Synonyms: |
GSA7 DKFZp434N0735 |
| Synonyms name: |
ubiquitin-activating enzyme E1-like protein |
| Locus: |
3p25, 3 |
| Discovery year: |
2003-11-20 |
| GenBank acession: |
AF094516 |
| Entrez gene record: |
10533 |
| Pubmed identfication: |
10233149 |
| RefSeq identity: |
NM_006395 |
| Classification: |
Ubiquitin like modifier activating enzymes Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000129740 |