| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ATG7 (APG7) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This ATG7 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95352 |
| Purity: |
Antigen affinity |
| Description: |
Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine, Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes, Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress, Plays also a key role in the maintenance of axonal homeostasis, Required for autophagic death induced by caspase-8 inhibition, Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production, [UniProt], as well as in adipose differentiation, the formation of Paneth cell granules, the maintenance of hematopoietic stem cells, the prevention of axonal degeneration, ATG7 is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy |
| Immunogen: |
A portion of amino acids 22-51 from the human protein was used as the immunogen for this ATG7 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ATG7 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ATG7 (APG7) |
| Short name: |
ATG7 Antibody (APG7) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ATG7 (Antibody to) (APG7) |
| Alternative technique: |
antibodies |
| Identity: |
16935 |
| Gene: |
ATG7 |
More about : ATG7 |
| Long gene name: |
autophagy related 7 |
| Synonyms gene: |
APG7L |
| Synonyms gene name: |
APG7 autophagy 7-like (S, cerevisiae) , cerevisiae) ATG7 autophagy related 7 homolog (S |
| Synonyms: |
GSA7 DKFZp434N0735 |
| Synonyms name: |
ubiquitin-activating enzyme E1-like protein |
| Locus: |
3p25, 3 |
| Discovery year: |
2003-11-20 |
| GenBank acession: |
AF094516 |
| Entrez gene record: |
10533 |
| Pubmed identfication: |
10233149 |
| RefSeq identity: |
NM_006395 |
| Classification: |
Ubiquitin like modifier activating enzymes Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000129740 |