| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LSD1 (Nuclear envelope marker) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the LSD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LSD1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O60341 |
| Purity: |
Purified |
| Description: |
Acts as a corepressor by mediating demethylation of H3K4me, Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed, Alone, Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, CDN7 and KRT8, Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, Demethylates and stabilizes the DNA methylase DNMT1, Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me, Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation, Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, May play a role in the repression of neuronal genes, Required for gastrulation during embryogenesis, Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7, The presence of PRKCB in ANDR-containing complexes, [UniProt], a number of genes implicated in multilineage blood cell development, a specific tag for epigenetic transcriptional activation, a specific tag for epigenetic transcriptional repression, a specific tag that prevents demethylation H3K4me, by being recruited to ANDR target genes and mediating demethylation of H3K9me, depending on the context, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity, prevents H3K4me demethylase activity of KDM1A, thereby acting as a coactivator or a corepressor, via histone deacetylase (HDAC) recruitment, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3 |
| Immunogen: |
A portion of amino acids 819-852 from the human protein was used as the immunogen for this LSD1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LSD1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene: |
KDM1A |
More about : KDM1A |
| Gene target: |
LSD1 envelope |
| Short name: |
LSD1 Antibody (Nuclear envelope ) |
| Technique: |
Component cell markers and DNA bp, Protein markers in kDA, antibodies against human proteins, antibodies for, markers are , Antibody, DNA sequences , chromosome , er genes or , genetic markers , species, that can be used to identify individuals or , with a known location on a |
| Alternative name: |
LSD1 (Antibody to) (Nuclear envelope detection labelled) |
| Alternative technique: |
antibodies |
| Identity: |
29079 |
| Long gene name: |
lysine demethylase 1A |
| Synonyms gene: |
AOF2 KDM1 |
| Synonyms gene name: |
amine oxidase (flavin containing) domain 2 lysine (K)-specific demethylase 1 |
| Synonyms: |
KIAA0601 BHC110 LSD1 |
| Locus: |
1p36, 12 |
| Discovery year: |
2004-02-26 |
| GenBank acession: |
AL031428 |
| Entrez gene record: |
23028 |
| Pubmed identfication: |
9628581 12493763 |
| RefSeq identity: |
NM_015013 |
| Classification: |
Lysine demethylases |
| Havana BLAST/BLAT: |
OTTHUMG00000003220 |