| Antigen detectable with this ELISA kit: |
Vitamin D Receptor (VDR) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
228ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Receptor (VDR), The concentration of Vitamin D Receptor (VDR) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Receptor (VDR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Vitamin D Receptor (VDR), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Vitamin D Receptor (VDR) |
| Short name: |
Vitamin D Receptor (VDR) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
25- dihydroxyvitamin D3) receptor) Enzyme-linked immunosorbent assay reagent, Vitamin D Receptor (vitamin D (1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006816 and calcium ion transport and biological process this GO :0006874 and cellular calcium ion homeostasis and biological process this GO :0007165 and signal transduction and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007595 and lactation and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008434 and vitamin D3 receptor activity and molecular function this GO :0009887 and organ morphogenesis and biological process this GO :0010467 and gene expression and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010839 and negative regulation of keratinocyte proliferation and biological process this GO :0010980 and positive regulation of vitamin D 24-hydroxylase activity and biological process this GO :0038183 and bile acid signaling pathway and biological process this GO :0038186 and lithocholic acid receptor activity and molecular function this GO :0043401 and steroid hormone mediated signaling pathway and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045618 and positive regulation of keratinocyte differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046697 and decidualization and biological process this GO :0046965 and retinoid X receptor binding and molecular function this GO :0050892 and intestinal absorption and biological process this GO :0060058 and positive regulation of apoptotic process involved in mammary gland involution and biological process this GO :0060558 and regulation of calcidiol 1-monooxygenase activity and biological process this GO :0060745 and mammary gland branching involved in pregnancy and biological process this GO :0070561 and vitamin D receptor signaling pathway and biological process this GO :0070644 and vitamin D response element binding and molecular function this GO :1902098 and calcitriol binding and molecular function this GO :1902121 and lithocholic acid binding and molecular function, NR1I1 and PPP1R163, VDR and IDBG-29237 and ENSG00000111424 and 7421, VDR and IDBG-633008 and ENSBTAG00000016414 and 533656, Vdr and IDBG-179227 and ENSMUSG00000022479 and 22337, lithocholic acid binding, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000902 and cell morphogenesis and biological process this GO :0001501 and skeletal system development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003707 and steroid hormone receptor activity and molecular function this GO :0004879 and ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003707 : steroid hormone receptor activity and also this GO :0004879 : ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0008434 : vitamin D3 receptor activity and also this GO :0038186 : lithocholic acid receptor activity and also this GO :0043565 : sequence-specific DNA binding and also this GO :0046965 : retinoid X receptor binding and also this GO :0070644 : vitamin D response element binding and also this GO :1902098 : calcitriol binding and also this GO :1902121 : lithocholic acid binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003707 : steroid hormone receptor activity, this GO :0004879 : ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0008434 : vitamin D3 receptor activity, this GO :0038186 : lithocholic acid receptor activity, this GO :0043565 : sequence-specific DNA binding, this GO :0046965 : retinoid X receptor binding, this GO :0070644 : vitamin D response element binding, this GO :1902098 : calcitriol binding, this GO :1902121 : lithocholic acid binding, 25- dihydroxyvitamin D3) receptor, vitamin D (1 |
| Identity: |
12679 |
| Gene: |
VDR |
More about : VDR |
| Long gene name: |
vitamin D (1, 25- dihydroxyvitamin D3) receptor |
| Synonyms: |
NR1I1 PPP1R163 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 163 |
| Locus: |
12q13, 11 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
J03258 |
| Entrez gene record: |
7421 |
| Pubmed identfication: |
1662663 |
| Classification: |
Nuclear hormone receptors Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000169890 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |