| Catalog number: | VPK-253 |
|---|---|
| Price: | 1382 € |
| Supplier: | Cellular biology labs |
| Product name: | RAPAd® miRNA Adenoviral Expression System |
| Quantity: | 1 test kit |
| Related search: |
| We send this reagant on: | Blue Ice |
|---|---|
| The temperatureerature for storage is: | -20º, C |
| SDS or safty data sheet: | 251, 252, 253, 254, 255, 256) GHS, more (VPK-250, pdf |
| Type of reagent: | Viral Expression |
| Related techniques: | miRNA Expression and Reporter Vectors, miRNA Viral Expression Vectors, microRNA Analysis |
| Use of the reagent: | Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, More recent technologies have shortened this time somewhat, Serial amplification of adenovirus produced using the RAPAd®, The RAPAd®, The system uses a backbone vector from which the 5' ITR, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification, packaging signal and E1 sequences have been removed, system does not significantly increase replication-competent adenovirus levels, while considerably shortening the production time to 2-3 weeks, Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination |
| Cellular use: | 000-fold fewer wild type plaques than most other expression systems, Engineered to produce 100- to 10 |
| Protocol information: | Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems |
| Reagent properties: | 2 kb, Cloning capacity of shuttle vector = 5 |
| Living modified organisme in this reagent or LMO: | Not applicable |
| Species compatible to: | Humans |
| Use of this test kit or product: | Genetics lab |
| Antigen: | a specific protein sequence |
| Buffer: | 73, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: | in kilo Danlto or kDa |
| This reagent is purified: | 97 |
| Gene target: | Adenoviral Expression, RAPAd® |
| Short name: | miRNA Adenoviral Expression System, RAPAd® |
| Technique: | microRNAs, miRNA |
| Alternative name: | RNA silencing Adenoviral Expression System, RAPAd® |
| Alternative technique: | micrornas |
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