| We send this reagant on: |
Blue Ice |
| The temperatureerature for storage is: |
-20º, C |
| SDS or safty data sheet: |
251, 252, 253, 254, 255, 256) GHS, more (VPK-250, pdf |
| Type of reagent: |
Viral Expression |
| Related techniques: |
Adenoviral Expression, Adenoviral Expression Systems, Viral Expression |
| Use of the reagent: |
Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, More recent technologies have shortened this time somewhat, Serial amplification of adenovirus produced using the RAPAd®, The RAPAd®, The RAPAd®, The system uses a backbone vector from which the 5' ITR, Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest , but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification, packaging signal and E1 sequences have been removed, system does not significantly increase replication-competent adenovirus levels, while considerably shortening the production time to 2-3 weeks, Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination, gene expression, to maximize |
| Cellular use: |
000-fold fewer wild type plaques than most other expression systems, Engineered to produce 100- to 10 |
| Protocol information: |
Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems |
| Reagent properties: |
Promoterless shuttle vector allows you to clone the optimal promoter with your gene of interest |
| Additional lab advice: |
5 kb, Cloning capacity of shuttle vector = 7 |
| Living modified organisme in this reagent or LMO: |
Not applicable |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
70, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Gene target: |
Universal Adenoviral Expression, RAPAd® |
| Short name: |
Universal Adenoviral Expression System, RAPAd® |
| Alternative name: |
Universal Adenoviral Expression System, RAPAd® |