| Antigen detectable with this ELISA kit: |
Stratifin (SFN) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 13 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cerebrospinal fluid and other biological fluids, plasma, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stratifin (SFN), The concentration of Stratifin (SFN) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stratifin (SFN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Stratifin (SFN), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Stratifin (SFN) |
| Short name: |
Stratifin (SFN) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Stratifin (stratifin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
SFN and IDBG-647781 and ENSBTAG00000009223 and 528453, SFN and IDBG-94738 and ENSG00000175793 and 2810, Sfn and IDBG-195282 and ENSMUSG00000047281 and 55948, YWHAS, nuclei, phosphoprotein binding, this GO :0000079 and regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0001836 and release of cytochrome c from mitochondria and biological process this GO :0003334 and keratinocyte development and biological process this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0006915 and apoptotic process and biological process this GO :0007165 and signal transduction and biological process this GO :0008426 and protein kinase C inhibitor activity and molecular function this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010482 and regulation of epidermal cell division and biological process this GO :0010839 and negative regulation of keratinocyte proliferation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0030216 and keratinocyte differentiation and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030659 and cytoplasmic vesicle membrane and cellular component this GO :0031424 and keratinization and biological process this GO :0043154 and negative regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043588 and skin development and biological process this GO :0045606 and positive regulation of epidermal cell differentiation and biological process this GO :0046827 and positive regulation of protein export from nucleus and biological process this GO :0051219 and phosphoprotein binding and molecular function this GO :0051726 and regulation of cell cycle and biological process this GO :0061024 and membrane organization and biological process this GO :0061436 and establishment of skin barrier and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071901 and negative regulation of protein serine/threonine kinase activity and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008426 : protein kinase C inhibitor activity and also this GO :0019901 : protein kinase binding and also this GO :0019904 : protein domain specific binding and also this GO :0051219 : phosphoprotein binding, this GO :0008426 : protein kinase C inhibitor activity, this GO :0019901 : protein kinase binding, this GO :0019904 : protein domain specific binding, this GO :0051219 : phosphoprotein binding, stratifin |
| Identity: |
10773 |
| Gene: |
SFN |
More about : SFN |
| Long gene name: |
stratifin |
| Synonyms: |
YWHAS |
| Synonyms name: |
14-3-3 sigma |
| Locus: |
1p36, 11 |
| Discovery year: |
1994-09-15 |
| GenBank acession: |
BC023552 |
| Entrez gene record: |
2810 |
| Pubmed identfication: |
8515476 |
| RefSeq identity: |
NM_006142 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000004093 |