| Antigen detectable with this ELISA kit: |
Leptin Receptor (LEPR) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
66ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin Receptor (LEPR), The concentration of Leptin Receptor (LEPR) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin Receptor (LEPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Leptin Receptor (LEPR), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
LEPR |
More about : LEPR |
| Gene target: |
Leptin Receptor (LEPR) |
| Short name: |
Leptin Receptor (LEPR) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Leptin Receptor (leptin receptor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD295 and LEP-R and LEPRD and OB-R and OBR, Extracellular, LEPR and IDBG-638266 and ENSBTAG00000005910 and 497205, LEPR and IDBG-99469 and ENSG00000116678 and 3953, Lepr and IDBG-167734 and ENSMUSG00000057722 and 16847, identical protein binding, this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0004896 and cytokine receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006112 and energy reserve metabolic process and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008203 and cholesterol metabolic process and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043235 and receptor complex and cellular component this GO :0045721 and negative regulation of gluconeogenesis and biological process this GO :0051346 and negative regulation of hydrolase activity and biological process, this GO :0004888 : transmembrane signaling receptor activity, this GO :0004888 : transmembrane signaling receptor activity and also this GO :0004896 : cytokine receptor activity and also this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding, this GO :0004896 : cytokine receptor activity, this GO :0005515 : protein binding, this GO :0042802 : identical protein binding, 785813, leptin receptor |
| Identity: |
6554 |
| Long gene name: |
leptin receptor |
| Synonyms: |
OBR CD295 |
| Locus: |
1p31, 3 |
| Discovery year: |
1996-05-09 |
| GenBank acession: |
U43168 |
| Entrez gene record: |
3953 |
| Pubmed identfication: |
8548812 8812446 |
| RefSeq identity: |
NM_002303 |
| Classification: |
CD molecules Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000009115 |
| Locus Specific Databases: |
LRG_283 |