| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPR71 |
| Specificity: |
This antibody reacts specifically with GPR71 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPR71/T1R2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A controversial fifth taste, A family of G protein coupled receptors are involved in taste perception, Artificial sweeteners bind to either Gbg or Gaq coupled T1Rs to activate PLCb2 and generate IP3 and DAG, Both pathways ultimately lead to neurotransmitter release, First, Sweet taste transduction is carried out by two pathways (2), T1R1, T1R2, The T1R family consists of three members, The T1R1 and T1R3 heterodimer recognizes L-amino-acids to perceive umami taste, The T1R2 and T1R3 heterodimer functions as a receptor for sweet taste, The mouse T1R3 gene maps to chromosome 4 near the Sac locus, There are four widely accepted categories of taste perception, These proteins form heterodimers, a primary determinant of sweet preference in mice, and T1R3 (1-4), and T2R, and acesulfame-K (1â, and fungiform taste papillae, and includes T1R, and it is expressed in a subset of taste cells in circumvallate, and recognizes several sweet-tasting molecules, and sour, bitter, dulcin, foliate, has also been described, known as umami or monosodium glutamate (MSG), saccharin, salty, such as sucrose, sucrose and other sugars activate Gas via the T1Rs, sweet, which activates adenylyl cyclase to generate cAMP, which alters the selectivity of the subunits (1-4), which is involved in bitter taste perception, which is involved in sweet and umami taste perception, &euro, &ldquo, 4), The sense of taste provides animals with valuable information about the quality and nutritional value of food |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
T1R2 TR2 |
| Also known as: |
GPR71 Polyclonal Antibody |
| Other name: |
Anti-GPR71 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPR71 |
| Short name: |
Anti-GPR71 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPR71 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14905 |
| Gene: |
TAS1R2 |
More about : TAS1R2 |
| Long gene name: |
taste 1 receptor member 2 |
| Synonyms gene: |
GPR71 |
| Synonyms gene name: |
G protein-coupled receptor 71 taste receptor, member 2 , type 1 |
| Locus: |
1p36, 13 |
| Discovery year: |
2001-03-21 |
| Entrez gene record: |
80834 |
| Classification: |
Taste 1 receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000002442 |