| Long name: |
FITC Conjugated, GPR71 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GPR71 |
| Specificity: |
This is a highly specific antibody against GPR71 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human GPR71/T1R2 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A controversial fifth taste, A family of G protein coupled receptors are involved in taste perception, Artificial sweeteners bind to either Gbg or Gaq coupled T1Rs to activate PLCb2 and generate IP3 and DAG, Both pathways ultimately lead to neurotransmitter release, First, Sweet taste transduction is carried out by two pathways (2), T1R1, T1R2, The T1R family consists of three members, The T1R1 and T1R3 heterodimer recognizes L-amino-acids to perceive umami taste, The T1R2 and T1R3 heterodimer functions as a receptor for sweet taste, The mouse T1R3 gene maps to chromosome 4 near the Sac locus, There are four widely accepted categories of taste perception, These proteins form heterodimers, a primary determinant of sweet preference in mice, and T1R3 (1-4), and T2R, and acesulfame-K (1â, and fungiform taste papillae, and includes T1R, and it is expressed in a subset of taste cells in circumvallate, and recognizes several sweet-tasting molecules, and sour, bitter, dulcin, foliate, has also been described, known as umami or monosodium glutamate (MSG), saccharin, salty, such as sucrose, sucrose and other sugars activate Gas via the T1Rs, sweet, which activates adenylyl cyclase to generate cAMP, which alters the selectivity of the subunits (1-4), which is involved in bitter taste perception, which is involved in sweet and umami taste perception, &euro, &ldquo, 4), The sense of taste provides animals with valuable information about the quality and nutritional value of food |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
T1R2 TR2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GPR71 Conjugated |
| Short name: |
FITC Conjugated, GPR71 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GPR71 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
14905 |
| Gene: |
TAS1R2 |
More about : TAS1R2 |
| Long gene name: |
taste 1 receptor member 2 |
| Synonyms gene: |
GPR71 |
| Synonyms gene name: |
G protein-coupled receptor 71 taste receptor, member 2 , type 1 |
| Locus: |
1p36, 13 |
| Discovery year: |
2001-03-21 |
| Entrez gene record: |
80834 |
| Classification: |
Taste 1 receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000002442 |