| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
p150 CAF1 |
| Specificity: |
This antibody reacts specifically with p150 CAF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human p150 CAF1 |
| Gene ID: |
10036 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, Assembles histone octamers onto replicating DNA in vitro, CAF-1 performs the first step of the nucleosome assembly process, CHAF1A binds to histones H3 and H4, It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity), The CCR4-NOT complex functions as general transcription regulation complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), a complex thought to mediate chromatin assembly in DNA replication and DNA repair, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer, which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene, Core component of the CAF-1 complex |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
p150 CAF1 Polyclonal Antibody |
| Other name: |
Anti-p150 CAF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
p150 CAF1 |
| Short name: |
Anti-p150 CAF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-p150 CAF1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14101 |
| Gene: |
CNOT7 |
More about : CNOT7 |
| Long gene name: |
CCR4-NOT transcription complex subunit 7 |
| Synonyms gene: |
CAF1 |
| Synonyms gene name: |
CCR4-NOT transcription complex, subunit 7 |
| Synonyms name: |
BTG1 binding factor 1 |
| Locus: |
8p22 |
| Discovery year: |
2000-12-01 |
| GenBank acession: |
L46722 |
| Entrez gene record: |
29883 |
| Pubmed identfication: |
10637334 1538749 17264152 |
| RefSeq identity: |
NM_013354 |
| Classification: |
CCR4-NOT transcription complex |
| Havana BLAST/BLAT: |
OTTHUMG00000096971 |