| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SMURF1 |
| Specificity: |
This antibody reacts specifically with SMURF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SMURF1 |
| Gene ID: |
57154 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
2 receptor-regulated SMADs specific for the BMP pathway, Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members, E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA1625 |
| Also known as: |
SMURF1 Polyclonal Antibody |
| Other name: |
Anti-SMURF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SMURF1 |
| Short name: |
Anti-SMURF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SMAD specific E3 ubiquitin protein ligase 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
R-SMAD binding, SMURF1 and IDBG-28871 and ENSG00000198742 and 57154, SMURF1 and IDBG-640859 and ENSBTAG00000007118 and, Smurf1 and IDBG-208404 and ENSMUSG00000038780 and 75788, nuclei, this GO :0000209 and protein polyubiquitination and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006611 and protein export from nucleus and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007398 and ectoderm development and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030154 and cell differentiation and biological process this GO :0030279 and negative regulation of ossification and biological process this GO :0030424 and axon and cellular component this GO :0030509 and BMP signaling pathway and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0030579 and ubiquitin-dependent SMAD protein catabolic process and biological process this GO :0032801 and receptor catabolic process and biological process this GO :0034394 and protein localization to cell surface and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0043025 and neuronal cell body and cellular component this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0048185 and activin binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0016874 : ligase activity and also this GO :0048185 : activin binding and also this GO :0070411 : I-SMAD binding and also this GO :0070412 : R-SMAD binding, this GO :0005515 : protein binding, this GO :0016874 : ligase activity, this GO :0048185 : activin binding, this GO :0070411 : I-SMAD binding, this GO :0070412 : R-SMAD binding, SMAD specific E3 ubiquitin protein ligase 1 |
| Identity: |
16807 |
| Gene: |
SMURF1 |
More about : SMURF1 |
| Long gene name: |
SMAD specific E3 ubiquitin protein ligase 1 |
| Locus: |
7q22, 1 |
| Discovery year: |
2004-07-29 |
| GenBank acession: |
AB046845 |
| Entrez gene record: |
57154 |
| Pubmed identfication: |
10458166 |
| RefSeq identity: |
NM_020429 |
| Havana BLAST/BLAT: |
OTTHUMG00000150272 |