| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MARCH2 |
| Specificity: |
This antibody reacts specifically with 37316 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MARCH2 |
| Gene ID: |
51257 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Expressed in a variety of tissues, MARCH2 (membrane-associated ring finger (C3HC4) 2), MARCH2 functions as an E3 ubiquitin-protein ligase that is thought to mediate the ubiquitination and subsequent degradation of CD71 and B7-2 and may be involved in endosomal protein trafficking, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), also known as RNF172 or HSPC240, is a 246 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and contains one RING-CH-type zinc finger, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HSPC240 MARCH-II RNF172 |
| Also known as: |
MARCH2 Polyclonal Antibody |
| Other name: |
Anti-MARCH2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MARCH2 |
| Short name: |
Anti-MARCH2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, E3 ubiquitin protein ligase (polyclonal), antibody to-membrane-associated ring finger (C3HC4) 2 |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
E3 ubiquitin protein ligase, MARCH2 and IDBG-24762 and ENSG00000099785 and 51257, MARCH2 and IDBG-643827 and ENSBTAG00000021036 and 508949, March2 and IDBG-251885 and ENSMUSG00000079557 and 224703, Plasma membranes, metal ion binding, this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005765 and lysosomal membrane and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006897 and endocytosis and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0010008 and endosome membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0046872 and metal ion binding and molecular function, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0046872 : metal ion binding, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0046872 : metal ion binding, membrane-associated ring finger (C3HC4) 2 |
| Identity: |
28038 |
| Gene: |
MARCH2 |
More about : MARCH2 |
| Long gene name: |
membrane associated ring-CH-type finger 2 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , membrane-associated ring finger (C3HC4) 2 membrane-associated ring finger (C3HC4) 2 |
| Locus: |
19p13, 2 |
| Discovery year: |
2005-01-26 |
| GenBank acession: |
AF151074 |
| Entrez gene record: |
51257 |
| Pubmed identfication: |
11042152 14722266 |
| RefSeq identity: |
NM_016496 |
| Classification: |
Membrane associated ring-CH-type fingers Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000182282 |