| Long name: |
FITC Conjugated, MARCH2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
37316 |
| Specificity: |
This is a highly specific antibody against 37316 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human MARCH2 |
| Gene ID number: |
51257 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Expressed in a variety of tissues, MARCH2 (membrane-associated ring finger (C3HC4) 2), MARCH2 functions as an E3 ubiquitin-protein ligase that is thought to mediate the ubiquitination and subsequent degradation of CD71 and B7-2 and may be involved in endosomal protein trafficking, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), also known as RNF172 or HSPC240, is a 246 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and contains one RING-CH-type zinc finger, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HSPC240 MARCH-II RNF172 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MARCH2 Conjugated |
| Short name: |
FITC Conjugated, MARCH2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
E3 ubiquitin protein ligase (Antibody to), fluorecein coupled, membrane-associated ring finger (C3HC4) 2 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
E3 ubiquitin protein ligase, MARCH2 and IDBG-24762 and ENSG00000099785 and 51257, MARCH2 and IDBG-643827 and ENSBTAG00000021036 and 508949, March2 and IDBG-251885 and ENSMUSG00000079557 and 224703, Plasma membranes, metal ion binding, this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005765 and lysosomal membrane and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006897 and endocytosis and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0010008 and endosome membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0046872 and metal ion binding and molecular function, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0046872 : metal ion binding, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0046872 : metal ion binding, membrane-associated ring finger (C3HC4) 2 |
| Identity: |
28038 |
| Gene: |
MARCH2 |
More about : MARCH2 |
| Long gene name: |
membrane associated ring-CH-type finger 2 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , membrane-associated ring finger (C3HC4) 2 membrane-associated ring finger (C3HC4) 2 |
| Locus: |
19p13, 2 |
| Discovery year: |
2005-01-26 |
| GenBank acession: |
AF151074 |
| Entrez gene record: |
51257 |
| Pubmed identfication: |
11042152 14722266 |
| RefSeq identity: |
NM_016496 |
| Classification: |
Membrane associated ring-CH-type fingers Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000182282 |