| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPCR TGR5/GPBAR1 |
| Specificity: |
This antibody reacts specifically with GPBAR1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPBAR1 |
| Gene ID: |
151306 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Bile acids activate mitogen-activated protein (MAP) kinase pathways, Bile acids also provoke TGR5 to suppress macrophage functions, TGR5 is sensitive to bile acids and responds through a significant mechanism that coordinates energy homeostasis, TGR5-controlled signaling pathways may be good candidates for drug targets to treat common metabolic diseases, and atherosclerosis, and cause rapid intracellular cAMP production, and leukocytes, but not in brain, colon (without mucosa), hyperlipidemia, kidney, liver, or lung, placenta, promote an increase of guanosine 5'-O-3-thio-triphosphate binding in membrane fractions, skeletal muscle, small intestine, specifically induce TGR5 internalization, spleen, such as obesity, thymus, type II diabetes, The G protein-coupled receptor TGR5 is a 330-amino acid protein that is almost universally expressed in human tissues including heart |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BG37 GPCR TGR5 M-BAR GPCR19 GPR131 MGC40597 |
| Also known as: |
GPBAR1 Polyclonal Antibody |
| Other name: |
Anti-GPBAR1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPBAR1 |
| Short name: |
Anti-GPBAR1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPBAR1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
19680 |
| Gene: |
GPBAR1 |
More about : GPBAR1 |
| Long gene name: |
G protein-coupled bile acid receptor 1 |
| Locus: |
2q35 |
| Discovery year: |
2003-01-24 |
| GenBank acession: |
AB086170 |
| Entrez gene record: |
151306 |
| Pubmed identfication: |
12419312 |
| RefSeq identity: |
NM_001077191 |
| Classification: |
G protein-coupled bile acid receptor |
| Havana BLAST/BLAT: |
OTTHUMG00000155203 |