| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
KLHL13 |
| Specificity: |
This antibody reacts specifically with KLHL13 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human KLHL13 |
| Gene ID: |
90293 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
E3 ligases accept a ubiquitin residue from an E2 ubiquitin conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation, Expressed predominantly in brain, KLHL13 interacts with KLHL9 and CUL3, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate specific adapter of an E3 ubiquitin protein ligase complex, KLHL13 is thought to play a role in spatially orientating substrates in the CUL3 ligase, Specifically, Via its BTB and C terminal Kelch (BACK) motif, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin mediated proteolysis, also known as BKLHD2 (BTB and kelch domain containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain, KLHL13 (kelch like 13) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ10262 |
| Also known as: |
KLHL13 Polyclonal Antibody |
| Other name: |
Anti-KLHL13 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
KLHL13 |
| Short name: |
Anti-KLHL13 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-kelch-like 13 (Drosophila) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
KLHL13 and IDBG-629488 and ENSBTAG00000000501 and 528138, KLHL13 and IDBG-83132 and ENSG00000003096 and 90293, Klhl13 and IDBG-136215 and ENSMUSG00000036782 and 67455, multiple, protein binding, this GO :0000910 and cytokinesis and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0007067 and mitotic nuclear division and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0031463 and Cul3-RING ubiquitin ligase complex and cellular component, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, kelch-like 13 (Drosophila) |
| Identity: |
22931 |
| Gene: |
KLHL13 |
More about : KLHL13 |
| Long gene name: |
kelch like family member 13 |
| Synonyms gene: |
BKLHD2 KIAA1309 |
| Synonyms gene name: |
BTB and kelch domain containing 2, KIAA1309 kelch-like 13 (Drosophila) |
| Locus: |
Xq24 |
| Discovery year: |
2003-07-23 |
| GenBank acession: |
AB037730 |
| Entrez gene record: |
90293 |
| Pubmed identfication: |
10718198 |
| RefSeq identity: |
NM_033495 |
| Classification: |
BTB domain containing Kelch like |
| Havana BLAST/BLAT: |
OTTHUMG00000022252 |
| Locus Specific Databases: |
Mental Retardation database |