| Long name: |
FITC Conjugated, KLHL13 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KLHL13 |
| Specificity: |
This is a highly specific antibody against KLHL13 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human KLHL13 |
| Gene ID number: |
90293 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
E3 ligases accept a ubiquitin residue from an E2 ubiquitin conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation, Expressed predominantly in brain, KLHL13 interacts with KLHL9 and CUL3, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate specific adapter of an E3 ubiquitin protein ligase complex, KLHL13 is thought to play a role in spatially orientating substrates in the CUL3 ligase, Specifically, Via its BTB and C terminal Kelch (BACK) motif, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin mediated proteolysis, also known as BKLHD2 (BTB and kelch domain containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain, KLHL13 (kelch like 13) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
FLJ10262 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KLHL13 Conjugated |
| Short name: |
FITC Conjugated, KLHL13 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, kelch-like 13 (Drosophila) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
KLHL13 and IDBG-629488 and ENSBTAG00000000501 and 528138, KLHL13 and IDBG-83132 and ENSG00000003096 and 90293, Klhl13 and IDBG-136215 and ENSMUSG00000036782 and 67455, multiple, protein binding, this GO :0000910 and cytokinesis and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0007067 and mitotic nuclear division and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0031463 and Cul3-RING ubiquitin ligase complex and cellular component, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, kelch-like 13 (Drosophila) |
| Identity: |
22931 |
| Gene: |
KLHL13 |
More about : KLHL13 |
| Long gene name: |
kelch like family member 13 |
| Synonyms gene: |
BKLHD2 KIAA1309 |
| Synonyms gene name: |
BTB and kelch domain containing 2, KIAA1309 kelch-like 13 (Drosophila) |
| Locus: |
Xq24 |
| Discovery year: |
2003-07-23 |
| GenBank acession: |
AB037730 |
| Entrez gene record: |
90293 |
| Pubmed identfication: |
10718198 |
| RefSeq identity: |
NM_033495 |
| Classification: |
BTB domain containing Kelch like |
| Havana BLAST/BLAT: |
OTTHUMG00000022252 |
| Locus Specific Databases: |
Mental Retardation database |