| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DNase I |
| Specificity: |
This antibody reacts specifically with DNase I |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DNase I |
| Gene ID: |
1773 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis, A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, DNase I attacks each strand of DNA independently and the cleavage sites are random, DNase I is a Ca2+ and Mg2+ dependant endonuclease, DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides, DNase I is synthesized in the pancreas and stored in zymogen granules, In the form of a bovine pancreatic enzyme preparation, In the presence of Mg ion, It has been used to reduce the viscosity of cystic fibrosis sputum, The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides, and it was the first DNase for which a specific protein inhibitor was characterized, cells susceptible to apoptosis, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, 2 kb long with 9 exons separated by 8 introns, Deoxyribonuclease I gene is approximately 3 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DNAS1L3 LSD |
| Also known as: |
DNase I Polyclonal Antibody |
| Other name: |
Anti-DNase I Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DNase I |
| Short name: |
Anti-DNase I (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-DNase I (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2959 |
| Gene: |
DNASE1L3 |
More about : DNASE1L3 |
| Long gene name: |
deoxyribonuclease 1 like 3 |
| Synonyms gene name: |
deoxyribonuclease I like 3 |
| Synonyms name: |
DNase gamma |
| Locus: |
3p14, 3 |
| Discovery year: |
1997-05-15 |
| GenBank acession: |
AF047354 |
| Entrez gene record: |
1776 |
| Pubmed identfication: |
9205125 9714828 14646506 |
| RefSeq identity: |
NM_004944 |
| Havana BLAST/BLAT: |
OTTHUMG00000159153 |