| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
USP28 |
| Specificity: |
This antibody reacts specifically with USP28 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human USP28 |
| Gene ID: |
57646 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also involved in G2 DNA damage checkpoint, In contrast, In contrast, Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN, Specifically deubiquitinates MYC in the nucleoplasm, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C), by deubiquitinating CLSPN, it does not deubiquitinate PLK1, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex, Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA1515 |
| Also known as: |
USP28 Polyclonal Antibody |
| Other name: |
Anti-USP28 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
USP28 |
| Short name: |
Anti-USP28 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ubiquitin specific peptidase 28 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, USP28 and IDBG-71844 and ENSG00000048028 and 57646, Usp28 and IDBG-162407 and ENSMUSG00000032267 and 235323, nuclei, this GO :0000077 and DNA damage checkpoint and biological process this GO :0004221 and ubiquitin thiolesterase activity and molecular function this GO :0004843 and ubiquitin-specific protease activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0006281 and DNA repair and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008234 and cysteine-type peptidase activity and molecular function this GO :0008283 and cell proliferation and biological process this GO :0010212 and response to ionizing radiation and biological process this GO :0016579 and protein deubiquitination and biological process this GO :0034644 and cellular response to UV and biological process this GO :0036459 and ubiquitinyl hydrolase activity and molecular function this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0043234 and protein complex and cellular component, this GO :0004221 : ubiquitin thiolesterase activity, this GO :0004221 : ubiquitin thiolesterase activity and also this GO :0004843 : ubiquitin-specific protease activity and also this GO :0005515 : protein binding and also this GO :0008234 : cysteine-type peptidase activity and also this GO :0036459 : ubiquitinyl hydrolase activity, this GO :0004843 : ubiquitin-specific protease activity, this GO :0005515 : protein binding, this GO :0008234 : cysteine-type peptidase activity, this GO :0036459 : ubiquitinyl hydrolase activity, ubiquitinyl hydrolase activity, 24505 and IDBG-630659 and ENSBTAG00000002323 and 508902, ubiquitin specific peptidase 28 |
| Identity: |
12625 |
| Gene: |
USP28 |
More about : USP28 |
| Long gene name: |
ubiquitin specific peptidase 28 |
| Synonyms gene name: |
ubiquitin specific protease 28 |
| Locus: |
11q23, 2 |
| Discovery year: |
2000-05-26 |
| GenBank acession: |
AB040948 |
| Entrez gene record: |
57646 |
| Pubmed identfication: |
12838346 11597335 |
| Classification: |
Ubiquitin specific peptidases |
| Havana BLAST/BLAT: |
OTTHUMG00000168205 |