| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Separase Ser1073 |
| Specificity: |
This antibody reacts specifically with Separase (Ser1073) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser1073 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073 |
| Gene ID: |
9700 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As the cellular division process continues, Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, Each cell must receive one chromatid of every chromosome, It not only cleaves cohesin at the onset of anaphase but also cleaves itself, Separase activity is highly regulated, Should a human cell become an aneuploid (one too many or too few chromatids), Should the embryo survive, allowing the chromatids to separate and divide with the cell, during mitosis, it will most likely develop severe birth defects or later develop malignant cancers, making certain that genomic information is replicated accurately, promoting downregulation of separase after anaphase, separase destroys cohesin by means of cleavage, the embryo most likely will not survive, Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ESP 1, ESP-1, ESP1, ESPL 1, ESPL-1, ESPL1, ESPL1_HUMAN, Extra spindle poles like 1, Extra spindle poles like 1 protein, SSE, Separase, Separin, Similar to fission yeast cut1and gene, Caspase like protein ESPL1 |
| Also known as: |
Separase (Ser1073) Polyclonal Antibody |
| Other name: |
Anti-Separase (Ser1073) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Separase (Ser1073) |
| Short name: |
Anti-Separase (Ser1073) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Separase (Ser1073) (polyclonal) |
| Alternative technique: |
polyclonals |