| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Separase |
| Specificity: |
This antibody reacts specifically with Separase |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Separase |
| Gene ID: |
9700 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
2009 [PubMed 19345191]), In vertebrates, MIM 606462) by ESPL1, Nov 2010], The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex, The second step involves cleavage of the cohesin subunit SCC1 (RAD21, or separase, sister chromatid cohesion is released in 2 steps via distinct mechanisms, which initiates the final separation of sister chromatids (Sun et al, Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance, [supplied by OMIM |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA0165 ESP1 SEPA |
| Also known as: |
Separase Polyclonal Antibody |
| Other name: |
Anti-Separase Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Separase |
| Short name: |
Anti-Separase (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Separase (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16856 |
| Gene: |
ESPL1 |
More about : ESPL1 |
| Long gene name: |
extra spindle pole bodies like 1, separase |
| Synonyms gene name: |
cerevisiae) , cerevisiae) extra spindle pole bodies homolog 1 (S, extra spindle poles like 1 (S |
| Synonyms name: |
cysteine protease , separin separase separin |
| Locus: |
12q13, 13 |
| Discovery year: |
2002-01-03 |
| GenBank acession: |
D79987 |
| Entrez gene record: |
9700 |
| Pubmed identfication: |
8724849 16258266 |
| RefSeq identity: |
NM_012291 |
| Havana BLAST/BLAT: |
OTTHUMG00000169674 |