| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
eIF2 alpha subunit 1 Ser52 |
| Specificity: |
This antibody reacts specifically with eIF2 alpha subunit 1 (Ser52) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser52 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR] |
| Gene ID: |
1965 |
| Swiss Prot: |
P05198 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In order for eIF-2 to recycle and catalyze another round of initiation, Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex, This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B, Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EIF-2, EIF-2A, EIF-2alpha, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2 |
| Also known as: |
eIF2 alpha subunit 1 (Ser52) Antibody |
| Other name: |
Anti-eIF2 alpha subunit 1 (Ser52) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
eIF2 alpha subunit 1 (Ser52) |
| Short name: |
Anti-eIF2 alpha subunit 1 (Ser52) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eIF2 a functionnal sequence 1 (Ser52) |