| Long name: |
Biotin Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
eIF2 alpha subunit 1 Ser52 |
| Specificity: |
This is a highly specific antibody against eIF2 alpha subunit 1 Ser52 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser52 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
50-91/315 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR] |
| Gene ID number: |
1965 |
| Swiss Prot: |
P05198 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
In order for eIF-2 to recycle and catalyze another round of initiation, Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex, This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B, Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
EIF-2, EIF-2A, EIF-2alpha, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eIF2 alpha subunit 1 (Ser52) Conjugated |
| Short name: |
Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, eIF2 a functionnal sequence 1 (Ser52) (Antibody to) |
| Alternative technique: |
antibodies |