| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MAP 1A/1B LC3 Alpha/Beta |
| Specificity: |
This antibody reacts specifically with MAP 1A/1B LC3 A/B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MAP LC3 Alpha |
| Gene ID: |
54901, 84557 |
| Swiss Prot: |
Q9GZQ8, Q9H492 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is expressed primarily in heart, LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3â, LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3â, MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles, MAP-LC3M Beta, MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 alpha) are subunits of both MAP1A and MAP1B, This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation, Two forms of LC3 Beta, a homolog of Apg8p, are produced post-translationally, brain and skeletal muscle, followed by the conversion of a fraction of LC3-I into LC3-II, is essential for autophagy and associated to the autophagosome membranes after processing, testis, the cytosolic LC3-I and the membrane-bound LC3-II, untranslated region of fibronectin mRNA, &circ, &euro, «, &trade, Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MAP1BLC3 MAP1ALC3 LC3 LC3A ATG8E |
| Also known as: |
MAP 1A/1B LC3 A/B Polyclonal Antibody |
| Other name: |
Anti-MAP 1A/1B LC3 A/B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MAP 1A/1B LC3 A/B |
| Short name: |
Anti-MAP 1A/1B LC3 A/B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-mitogen-stimulated protein kinase 1A/1B LC3 A/B (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6838 |
| Gene: |
MAP1LC3A |
More about : MAP1LC3A |
| Long gene name: |
microtubule associated protein 1 light chain 3 alpha |
| Locus: |
20q11, 22 |
| Discovery year: |
2000-06-29 |
| Entrez gene record: |
84557 |
| Pubmed identfication: |
8833088 17580304 |
| RefSeq identity: |
NM_181509 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000032306 |