| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HAUSP |
| Specificity: |
This antibody reacts specifically with HAUSP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HAUSP/USP7 |
| Gene ID: |
7874 |
| Swiss Prot: |
Q93009 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection, DNMT1, Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity, ERCC6, Exhibits a preference towards 'Lys-48'-linked ubiquitin chains, In association with DAXX, Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222), Involved in cell proliferation during early embryonic development, Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, MDM2, PTEN and DAXX, Together with DAXX, UHRF1, and also induces p53/TP53-dependent cell growth repression and apoptosis, both processes that are counteracted by PML, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, p53/TP53, preventing UV-induced degradation of ERCC6, preventing their degradation and promoting DNA methylation by DNMT1, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation, Hydrolase that deubiquitinates target proteins such as FOXO4 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
HAUSP Polyclonal Antibody |
| Other name: |
Anti-HAUSP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HAUSP |
| Short name: |
Anti-HAUSP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HAUSP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12630 |
| Gene: |
USP7 |
More about : USP7 |
| Long gene name: |
ubiquitin specific peptidase 7 |
| Synonyms gene: |
HAUSP |
| Synonyms gene name: |
ubiquitin specific peptidase 7 (herpes virus-associated) |
| Locus: |
16p13, 2 |
| Discovery year: |
1998-10-12 |
| GenBank acession: |
Z72499 |
| Entrez gene record: |
7874 |
| Pubmed identfication: |
12838346 9925944 |
| Classification: |
Ubiquitin specific peptidases |
| Havana BLAST/BLAT: |
OTTHUMG00000176903 |