| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PPAR alpha Ser12 |
| Specificity: |
This antibody reacts specifically with PPAR alpha (Ser12) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser12 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12 |
| Gene ID: |
5465 |
| Swiss Prot: |
Q07869 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activated by oleylethanolamide, Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), Functions as transcription activator for the ACOX1 and P450 genes, Key regulator of lipid metabolism, May be required for the propagation of clock information to metabolic pathways regulated by PER2, Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids, Regulates the peroxisomal beta-oxidation pathway of fatty acids, Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2, a naturally occurring lipid that regulates satiety, Ligand-activated transcription factor |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hPPAR NR1C1 |
| Also known as: |
PPAR alpha (Ser12) Polyclonal Antibody |
| Other name: |
Anti-PPAR alpha (Ser12) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PPAR alpha (Ser12) |
| Short name: |
Anti-PPAR alpha (Ser12) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PPAR a (Ser12) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9232 |
| Gene: |
PPARA |
More about : PPARA |
| Long gene name: |
peroxisome proliferator activated receptor alpha |
| Synonyms gene: |
PPAR |
| Synonyms gene name: |
alpha peroxisome proliferator-activated receptor alpha , peroxisome proliferative activated receptor |
| Locus: |
22q13, 31 |
| Discovery year: |
1993-11-01 |
| GenBank acession: |
L02932 |
| Entrez gene record: |
5465 |
| Pubmed identfication: |
7684926 10591208 |
| RefSeq identity: |
NM_001001928 |
| Classification: |
Nuclear hormone receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000150443 |