| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TrkB Tyr706 + Tyr707, TrkA Tyr680 + Tyr681 |
| Specificity: |
These sites in TrkB are homologous to those of Tyr705 and Tyr706 in Mouse and Rat, The phosphorylation sites Tyr680 and Tyr681 in TrkA are homologous to those of Tyr683 and Tyr683 in Mouse and Rat, TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr680+ Tyr681 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human TrkB around the phosphorylation site of Tyr680/681 |
| Gene ID: |
4914 |
| Swiss Prot: |
P04629 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation, FRS2, High affinity receptor for NGF which is its primary ligand, However, In absence of ligand and activation, Isoform TrkA-III is resistant to NGF, Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival, Recruits, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation, Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival, Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival, Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival, Upon dimeric NGF ligand-binding, autophosphorylation and activation, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade, differentiation and survival of sympathetic and nervous neurons, it can also bind and be activated by NTF3/neurotrophin-3, making the survival of neurons dependent on trophic factors, may promote cell death, phosphorylates and/or activates several downstream effectors including SHC1, undergoes homodimerization, Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
High affinity nerve growth factor receptor, NTRK1, Neurotrophic tyrosine kinase receptor type 1, TRK, TRK1, TRK1-transforming tyrosine kinase protein, TRKA, Trk-A, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, MTC |
| Also known as: |
TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody |
| Other name: |
Anti-TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) |
| Short name: |
Anti-TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TrkA (Tyr680 and Tyr681)/TrkB (Tyr706 and Tyr707) |