| Long name: |
Cy3 Conjugated, TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
TrkB Tyr706 + Tyr707, TrkA Tyr680 + Tyr681 |
| Specificity: |
The phosphorylation sites Tyr680 and Tyr681 in TrkA are homologous to those of Tyr683 and Tyr683 in Mouse and Rat, These sites in TrkB are homologous to those of Tyr705 and Tyr706 in Mouse and Rat, TrkB Tyr706 + Tyr707, This is a highly specific antibody against TrkA Tyr680 + Tyr681 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr680+ Tyr681 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
670-720/822 |
| Subcellular location: |
Cell membrane |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human TrkB around the phosphorylation site of Tyr680/681 |
| Gene ID number: |
4914 |
| Swiss Prot: |
P04629 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation, FRS2, High affinity receptor for NGF which is its primary ligand, However, In absence of ligand and activation, Isoform TrkA-III is resistant to NGF, Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival, Recruits, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation, Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival, Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival, Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival, Upon dimeric NGF ligand-binding, autophosphorylation and activation, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade, differentiation and survival of sympathetic and nervous neurons, it can also bind and be activated by NTF3/neurotrophin-3, making the survival of neurons dependent on trophic factors, may promote cell death, phosphorylates and/or activates several downstream effectors including SHC1, undergoes homodimerization, Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
High affinity nerve growth factor receptor, NTRK1, Neurotrophic tyrosine kinase receptor type 1, TRK, TRK1, TRK1-transforming tyrosine kinase protein, TRKA, Trk-A, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, MTC |
| Properties: |
1 of the TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Conjugated |
| Short name: |
Conjugated, TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, TrkA (Tyr680 and Tyr681)/TrkB (Tyr706 and Tyr707) (Antibody to) |
| Alternative technique: |
antibodies |