| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
c-Raf Ser259 |
| Specificity: |
This antibody reacts specifically with c-Raf (Ser259) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser259 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser259 |
| Gene ID: |
5894 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is part of the Ras dependent signaling pathway from receptors to the nucleus, Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, bRaf, cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, phosphorylates MEK which in turn phosphorylates ERK, The Raf family of serine/threonine specific kinases is comprised of three members (aRaf |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
6430402F14Rik, AA990557, BB129353, C-RAF, Craf 1 transforming gene, Craf1, Craf1 transforming gene, D830050J10Rik, EC 2, MGC102375, Murine sarcoma 3611 oncogene 1, RAF, RAF1, Raf 1, Raf proto oncogene serine/threonine protein kinase, Raf-1, Raf1Ser259, c Raf, c-Raf, c-Raf, cRaf, murine leukemia viral v-raf-1 oncogene homolog 1 3611-MSV, proto-oncogene c-RAF, v-Raf, v-raf-1 murine leukemia viral oncogene homolog 1, vraf1 murine leukemia viral oncogene homolog 1, 1, 7, 11, RAF1phospho S259 |
| Also known as: |
c-Raf (Ser259) Polyclonal Antibody |
| Other name: |
Anti-c-Raf (Ser259) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
c-Raf (Ser259) |
| Short name: |
Anti-c-Raf (Ser259) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-c-Raf (Ser259) (polyclonal) |
| Alternative technique: |
polyclonals |